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Article

Identification and Accumulation of Phenolic Compounds in the Leaves and Bark of Salix alba (L.) and Their Biological Potential

1
Department of Biology and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Lodz, Muszyńskiego 1, 90-151 Łódź, Poland
2
Department of Cosmetic Raw Materials Chemistry, Faculty of Pharmacy, Medical University of Lodz, Muszyńskiego 1, 90-151 Łódź, Poland
3
Department of Molecular Carcinogenesis, Medical University of Lodz, Żeligowskiego 7/9, 90-752 Łódź, Poland
4
Department of Fruit, Vegetable and Plant Nutraceuticals Technology, Wroclaw University of Environmental and Life Sciences, Chełmońskiego 37, 51-630 Wrocław, Poland
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(10), 1391; https://doi.org/10.3390/biom10101391
Received: 31 August 2020 / Revised: 16 September 2020 / Accepted: 28 September 2020 / Published: 29 September 2020
(This article belongs to the Collection Pharmacology of Medicinal Plants)
The study examines the phenolic compounds in hydromethanolic extracts of Salix alba (L.) leaves and bark as well as their antioxidant activity and cytotoxic potential. UPLC-PDA-Q/TOF-MS analysis showed a total of 29 phenolic compounds in leaves and 34 in bark. Total phenolic compound content was 5575.96 mg/100 g of dry weight (DW) in leaves and 2330.31 mg/100 g DW in bark. The compounds were identified as derivatives of phenolic acids (seven in leaves and five in bark), flavanols and procyanidins (eight in leaves and 26 in bark) and flavonols (14 in leaves and three in bark). Both extracts exhibited strong antioxidant potential, assessed by radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), but the bark extract was even stronger than the ascorbic acid used as a standard. The cytotoxicity of both extracts was evaluated against human skin fibroblasts and human epidermal keratinocytes cell lines using the Presto Blue cell viability assay. The keratinocytes were more resistant to tested extracts than fibroblasts. The leaf and bark extracts at concentrations which exhibited antioxidant activity were also not toxic against the keratinocyte cell line. Thus, S. alba extracts, especially the leaf extract, offer promise as a nontoxic natural antioxidant, in cosmetic products or herbal medicines, and as a source of bioactive secondary metabolites. View Full-Text
Keywords: polyphenolic compounds; antioxidant activity; cytotoxic activity; fibroblast cell line; keratinocyte cell line polyphenolic compounds; antioxidant activity; cytotoxic activity; fibroblast cell line; keratinocyte cell line
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MDPI and ACS Style

Piątczak, E.; Dybowska, M.; Płuciennik, E.; Kośla, K.; Kolniak-Ostek, J.; Kalinowska-Lis, U. Identification and Accumulation of Phenolic Compounds in the Leaves and Bark of Salix alba (L.) and Their Biological Potential. Biomolecules 2020, 10, 1391. https://doi.org/10.3390/biom10101391

AMA Style

Piątczak E, Dybowska M, Płuciennik E, Kośla K, Kolniak-Ostek J, Kalinowska-Lis U. Identification and Accumulation of Phenolic Compounds in the Leaves and Bark of Salix alba (L.) and Their Biological Potential. Biomolecules. 2020; 10(10):1391. https://doi.org/10.3390/biom10101391

Chicago/Turabian Style

Piątczak, Ewelina, Monika Dybowska, Elżbieta Płuciennik, Katarzyna Kośla, Joanna Kolniak-Ostek, and Urszula Kalinowska-Lis. 2020. "Identification and Accumulation of Phenolic Compounds in the Leaves and Bark of Salix alba (L.) and Their Biological Potential" Biomolecules 10, no. 10: 1391. https://doi.org/10.3390/biom10101391

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