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Article

Anti-Obesity Effects of Soybean Embryo Extract and Enzymatically-Modified Isoquercitrin

1
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Korea
2
College of Pharmacy, Pusan National University, Busan 46241, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Biomolecules 2020, 10(10), 1394; https://doi.org/10.3390/biom10101394
Received: 31 August 2020 / Revised: 25 September 2020 / Accepted: 29 September 2020 / Published: 30 September 2020
(This article belongs to the Collection Pharmacology of Medicinal Plants)
Soy isoflavones are bioactive phytoestrogens with known health benefits. Soybean embryo extract (SEE) has been consumed as a source of isoflavones, mainly daidzein, glycitein, and genistein. While previous studies have reported the anti-obesity effects of SEE, this study investigates their molecular mechanisms and the synergistic effects of co-treatment with SEE and enzymatically modified isoquercitrin (EMIQ). SEE upregulated genes involved in lipolysis and brown adipocyte markers and increased mitochondrial content in differentiated C3H10T1/2 adipocytes in vitro. Next, we use a high-fat diet-induced obesity mouse model to determine the anti-obesity effect of SEE. Two weeks of single or combined treatment with SEE and EMIQ significantly reduced body weight gain and improved glucose tolerance. Mechanistically, SEE treatment increased mitochondrial content and upregulated genes involved in lipolysis in adipose tissue through the cAMP/PKA-dependent signaling pathway. These effects required a cytosolic lipase adipose triglyceride lipase (ATGL) expression, confirmed by an adipocyte-specific ATGL knockout mouse study. Collectively, this study demonstrates that SEE exerts anti-obesity effects through the activation of adipose tissue metabolism and exhibits a synergistic effect of co-treatment with EMIQ. These results improve our understanding of the mechanisms underlying the anti-obesity effects of SEE related to adipose tissue metabolism. View Full-Text
Keywords: soy embryo extract; enzymatically-modified isoquercitrin; adipose tissue; anti-obesity; mitochondria; PKA signaling; ATGL soy embryo extract; enzymatically-modified isoquercitrin; adipose tissue; anti-obesity; mitochondria; PKA signaling; ATGL
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MDPI and ACS Style

Kim, M.; Im, S.; Cho, Y.k.; Choi, C.; Son, Y.; Kwon, D.; Jung, Y.-S.; Lee, Y.-H. Anti-Obesity Effects of Soybean Embryo Extract and Enzymatically-Modified Isoquercitrin. Biomolecules 2020, 10, 1394. https://doi.org/10.3390/biom10101394

AMA Style

Kim M, Im S, Cho Yk, Choi C, Son Y, Kwon D, Jung Y-S, Lee Y-H. Anti-Obesity Effects of Soybean Embryo Extract and Enzymatically-Modified Isoquercitrin. Biomolecules. 2020; 10(10):1394. https://doi.org/10.3390/biom10101394

Chicago/Turabian Style

Kim, Minsu, Seowoo Im, Yoon k. Cho, Cheoljun Choi, Yeonho Son, Doyoung Kwon, Young-Suk Jung, and Yun-Hee Lee. 2020. "Anti-Obesity Effects of Soybean Embryo Extract and Enzymatically-Modified Isoquercitrin" Biomolecules 10, no. 10: 1394. https://doi.org/10.3390/biom10101394

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