Aedes albopictus D7 Salivary Protein Prevents Host Hemostasis and Inflammation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sequence Alignment
2.2. Cloning, Expression, and Purification of AlboDL71
2.3. Isothermal Titration Calorimetry
2.4. AlboD7L1 Structure Modell Prediction
2.5. Ex Vivo Platelet Aggregation Assays
2.6. Animals and In Vivo Leukocyte Recruitment Experiment
3. Results
3.1. Initial Characterization of AlboD7L1
3.2. Binding Properties of AlboD7L1
3.3. AlboD7L1 Structure Modeling
3.4. AlboD7L1 Inhibits Platelet Aggregation
3.5. AlboD7L1 Prevents Leukocyte Recruitment In Vivo
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Ligand | KD (nM) ± SE | ΔH (kcal/mol) ± SE | −TΔS (kcal/mol) |
---|---|---|---|
Dopamine † | 11.0 ± 2.19 | −12.3 ± 0.197 | 1.23 |
Norepinephrine † | 3.67 ± 0.994 | −11.6 ± 0.186 | −0.116 |
Epinephrine † | 4110 ± 3330 | −6.83 ± 0.809 | −0.637 |
Histamine † | 278 ± 66.2 | −7.47 ± 0.378 | −1.63 |
Tryptamine † | 570 ± 97.7 | −6.43 ± 0.223 | −2.23 |
Serotonin ‡ | 4.51 ± 2.66 | −13.7 ± 0.445 | 2.13 |
LTB4 § | 342 ± 65.0 | −2.74 ± 0.085 | −6.23 |
LTC4 † | 67.7 ± 22.4 | −7.90 ± 0.350 | −2.05 |
LTD4 † | 332 ± 48.3 | −5.00 ± 0.139 | −3.98 |
LTE4 § | 567 ± 115 | −3.98 ± 0.180 | −4.69 |
U-46619 ‡ | 946 ± 269 | −8.54 ± 0.785 | 0.189 |
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Martin-Martin, I.; Smith, L.B.; Chagas, A.C.; Sá-Nunes, A.; Shrivastava, G.; Valenzuela-Leon, P.C.; Calvo, E. Aedes albopictus D7 Salivary Protein Prevents Host Hemostasis and Inflammation. Biomolecules 2020, 10, 1372. https://doi.org/10.3390/biom10101372
Martin-Martin I, Smith LB, Chagas AC, Sá-Nunes A, Shrivastava G, Valenzuela-Leon PC, Calvo E. Aedes albopictus D7 Salivary Protein Prevents Host Hemostasis and Inflammation. Biomolecules. 2020; 10(10):1372. https://doi.org/10.3390/biom10101372
Chicago/Turabian StyleMartin-Martin, Ines, Leticia Barion Smith, Andrezza Campos Chagas, Anderson Sá-Nunes, Gaurav Shrivastava, Paola Carolina Valenzuela-Leon, and Eric Calvo. 2020. "Aedes albopictus D7 Salivary Protein Prevents Host Hemostasis and Inflammation" Biomolecules 10, no. 10: 1372. https://doi.org/10.3390/biom10101372