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Open AccessArticle

General and Genomic DNA-Binding Specificity for the Thermus thermophilus HB8 Transcription Factor TTHB023

1
Department of Chemistry and Biochemistry, Kennesaw State University, Kennesaw, GA 30144, USA
2
Medical College of Georgia, Augusta University, Augusta, GA 30912, USA
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(1), 94; https://doi.org/10.3390/biom10010094
Received: 14 December 2019 / Revised: 2 January 2020 / Accepted: 3 January 2020 / Published: 6 January 2020
(This article belongs to the Special Issue 2019 Feature Papers by Biomolecules’ Editorial Board Members)
Transcription factors are proteins that recognize specific DNA sequences and affect local transcriptional processes. They are the primary means by which all organisms control specific gene expression. Understanding which DNA sequences a particular transcription factor recognizes provides important clues into the set of genes that they regulate and, through this, their potential biological functions. Insights may be gained through homology searches and genetic means. However, these approaches can be misleading, especially when comparing distantly related organisms or in cases of complicated transcriptional regulation. In this work, we used a biochemistry-based approach to determine the spectrum of DNA sequences specifically bound by the Thermus thermophilus HB8 TetR-family transcription factor TTHB023. The consensus sequence 5′–(a/c)Y(g/t)A(A/C)YGryCR(g/t)T(c/a)R(g/t)–3′ was found to have a nanomolar binding affinity with TTHB023. Analyzing the T. thermophilus HB8 genome, several TTHB023 consensus binding sites were mapped to the promoters of genes involved in fatty acid biosynthesis. Notably, some of these were not identified previously through genetic approaches, ostensibly given their potential co-regulation by the Thermus thermophilus HB8 TetR-family transcriptional repressor TTHA0167. Our investigation provides additional evidence supporting the usefulness of a biochemistry-based approach for characterizing putative transcription factors, especially in the case of cooperative regulation. View Full-Text
Keywords: DNA-binding specificity; REPSA; Thermus thermophilus HB8; TTHB023; transcriptional regulation DNA-binding specificity; REPSA; Thermus thermophilus HB8; TTHB023; transcriptional regulation
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Cox, J.S.; Van Dyke, M.W. General and Genomic DNA-Binding Specificity for the Thermus thermophilus HB8 Transcription Factor TTHB023. Biomolecules 2020, 10, 94.

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