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Macromolecular Crowding Increases the Affinity of the PHD of ING4 for the Histone H3K4me3 Mark

1
CIC bioGUNE, Bizkaia Science and Technology Park, bld 800, 48160 Derio, Bizkaia, Spain
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CIC bioGUNE, Bizkaia Science and Technology Park, bld 800, 48160 Derio, Bizkaia, Spain
3
IKERBASQUE, Basque Foundation for Science, Maria Diaz de Haro 3, 6 solairua, 48013 Bilbao, Bizkaia, Spain
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(2), 234; https://doi.org/10.3390/biom10020234
Received: 17 December 2019 / Revised: 27 January 2020 / Accepted: 30 January 2020 / Published: 4 February 2020
(This article belongs to the Special Issue 2019 Feature Papers by Biomolecules’ Editorial Board Members)
The five members of the family of tumor suppressors ING contain a Plant Homeodomain (PHD) that specifically recognizes histone H3 trimethylated at lysine 4 (H3K4me3) with an affinity in the low micromolar range. Here, we use NMR to show that in the presence of 15% Ficoll 70, an inert macromolecular crowding agent, the mode of binding does not change but the affinity increases by one order of magnitude. The affinity increases also for unmethylated histone H3 tail, but the difference with H3K4me3 is larger in the presence of Ficoll. These results indicate that in the cellular milieu, the affinity of the ING proteins for their chromatin target is larger than previously thought.
Keywords: macromolecular crowding; PHD; ING4; histone H3; H3K4me3; NMR; protein–protein interaction macromolecular crowding; PHD; ING4; histone H3; H3K4me3; NMR; protein–protein interaction
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Palacios, A.; Blanco, F.J. Macromolecular Crowding Increases the Affinity of the PHD of ING4 for the Histone H3K4me3 Mark. Biomolecules 2020, 10, 234.

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