Gas Chromatography–Mass Spectrometry-Based Metabolomic Analysis of Wagyu and Holstein Beef
Division of Livestock Feeding and Management, National Agriculture and Food Research Organization, Nasushiobara, Tochigi 329-2793, Japan
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Metabolites 2020, 10(3), 95; https://doi.org/10.3390/metabo10030095
Received: 6 February 2020
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Revised: 5 March 2020
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Accepted: 5 March 2020
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Published: 6 March 2020
(This article belongs to the Special Issue Metabolomic Applications in Animal Science)
Japanese Black cattle (Wagyu) beef is characterized by high intramuscular fat content and has a characteristic sweet taste. However, the chemical components for characterizing the sweet taste of Wagyu beef have been unclear. In this experiment, we conducted a metabolomic analysis of the longissimus muscle (sirloin) in Wagyu and Holstein cattle to determine the key components associated with beef taste using gas chromatography–mass spectrometry (GC-MS). Holstein sirloin beef was characterized by the abundance of components such as glutamine, ribose-5-phosphate, uric acid, inosine monophosphate, 5-oxoproline, and glycine. In contrast, Wagyu sirloin beef was characterized by the abundance of sugar components (maltose and xylitol). Dietary fat is known to increase the intensity of sweet taste. These results suggest that the sweet taste of Wagyu beef is due to the synergetic effects of higher sugar components and intramuscular fat.
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Keywords:
metabolome; beef; Wagyu; Holstein
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MDPI and ACS Style
Yamada, T.; Kamiya, M.; Higuchi, M. Gas Chromatography–Mass Spectrometry-Based Metabolomic Analysis of Wagyu and Holstein Beef. Metabolites 2020, 10, 95. https://doi.org/10.3390/metabo10030095
AMA Style
Yamada T, Kamiya M, Higuchi M. Gas Chromatography–Mass Spectrometry-Based Metabolomic Analysis of Wagyu and Holstein Beef. Metabolites. 2020; 10(3):95. https://doi.org/10.3390/metabo10030095
Chicago/Turabian StyleYamada, Tomoya, Mituru Kamiya, and Mikito Higuchi. 2020. "Gas Chromatography–Mass Spectrometry-Based Metabolomic Analysis of Wagyu and Holstein Beef" Metabolites 10, no. 3: 95. https://doi.org/10.3390/metabo10030095
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