Next Article in Journal
Sulfonamide Inhibition Profile of the β-Carbonic Anhydrase from Malassezia restricta, An Opportunistic Pathogen Triggering Scalp Conditions
Previous Article in Journal
Metabolomics in the Context of Plant Natural Products Research: From Sample Preparation to Metabolite Analysis
Open AccessArticle

Comparative Analysis of β-Carotene Production by Mucor circinelloides Strains CBS 277.49 and WJ11 under Light and Dark Conditions

1
Colin Ratledge Center of microbial lipids, Shandong University of Technology, Zibo 255000, China
2
Departamento de Genética y Microbiología (Unidad asociada al IQFR-CSIC) and Facultad de Biología, Universidad de Murcia, 30100 Murcia, Spain
*
Author to whom correspondence should be addressed.
Both authors contributed equally to the work.
Metabolites 2020, 10(1), 38; https://doi.org/10.3390/metabo10010038
Received: 13 December 2019 / Revised: 5 January 2020 / Accepted: 15 January 2020 / Published: 16 January 2020
Carotenoids are natural potent antioxidants and free radical scavengers which are able to modulate the pathogenesis of some cancers and heart diseases in human, indicating their importance in being provided through the diet. Mucor circinelloides accumulates β-carotene as the main carotenoid compound and has been used as a model organism in carotenogenic studies. In the present study, the potential of two M. circinelloides strains to accumulate β-carotene was investigated under light and dark conditions. The results, which were quantitated by HPLC, showed that CBS 277.49 accumulated higher pigment in comparison to WJ11 under both conditions. Continuous illumination triggered the pigment accumulation up to 2.7-fold in strain CBS 277.49 and 2.2-fold in strain WJ11 in comparison to dark. The mRNA analysis of the four key genes involved in isoprenoid pathway by RT-qPCR showed higher transcriptional levels in CBS 277.49 as compared to WJ11, indicating that the pigment production metabolic machinery is more active in CBS 277.49 strain. A new scope for further research was established by this work for improved β-carotene production in the high producing strain CBS 277.49. View Full-Text
Keywords: β-carotene; Mucor circinelloides strains; mevalonate pathway; transcriptional levels β-carotene; Mucor circinelloides strains; mevalonate pathway; transcriptional levels
Show Figures

Figure 1

MDPI and ACS Style

Naz, T.; Nosheen, S.; Li, S.; Nazir, Y.; Mustafa, K.; Liu, Q.; Garre, V.; Song, Y. Comparative Analysis of β-Carotene Production by Mucor circinelloides Strains CBS 277.49 and WJ11 under Light and Dark Conditions. Metabolites 2020, 10, 38.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop