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Metabolomics in the Context of Plant Natural Products Research: From Sample Preparation to Metabolite Analysis

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Department of Pharmacognosy, Faculty of Pharmacy, Menoufia University, Gamal Abd El Nasr st., Shibin Elkom, Menoufia 32511, Egypt
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Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany
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Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo 11751, Egypt
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Center of Plant Systems Biology and Biotechnology (CPSBB), 4000 Plovdiv, Bulgaria
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Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt
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Chemistry Department, School of Sciences & Engineering, The American University in Cairo, New Cairo 11835, Egypt
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Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza 11787, Egypt
*
Authors to whom correspondence should be addressed.
Metabolites 2020, 10(1), 37; https://doi.org/10.3390/metabo10010037 (registering DOI)
Received: 25 November 2019 / Revised: 25 December 2019 / Accepted: 11 January 2020 / Published: 15 January 2020
Plant-derived natural products have long been considered a valuable source of lead compounds for drug development. Natural extracts are usually composed of hundreds to thousands of metabolites, whereby the bioactivity of natural extracts can be represented by synergism between several metabolites. However, isolating every single compound from a natural extract is not always possible due to the complex chemistry and presence of most secondary metabolites at very low levels. Metabolomics has emerged in recent years as an indispensable tool for the analysis of thousands of metabolites from crude natural extracts, leading to a paradigm shift in natural products drug research. Analytical methods such as mass spectrometry (MS) and nuclear magnetic resonance (NMR) are used to comprehensively annotate the constituents of plant natural products for screening, drug discovery as well as for quality control purposes such as those required for phytomedicine. In this review, the current advancements in plant sample preparation, sample measurements, and data analysis are presented alongside a few case studies of the successful applications of these processes in plant natural product drug discovery. View Full-Text
Keywords: metabolomics; plant natural products; drug discovery; metabolite extraction; liquid chromatography; gas chromatography; mass spectrometry; NMR metabolomics; plant natural products; drug discovery; metabolite extraction; liquid chromatography; gas chromatography; mass spectrometry; NMR
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Salem, M.A.; Perez de Souza, L.; Serag, A.; Fernie, A.R.; Farag, M.A.; Ezzat, S.M.; Alseekh, S. Metabolomics in the Context of Plant Natural Products Research: From Sample Preparation to Metabolite Analysis. Metabolites 2020, 10, 37.

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