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Open AccessArticle

On the Inverse Correlation of Protein and Oil: Examining the Effects of Altered Central Carbon Metabolism on Seed Composition Using Soybean Fast Neutron Mutants

1
Donald Danforth Plant Science Center, St. Louis, MO 63132, USA
2
Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS 66506, USA
3
United States Department of Agriculture, Agricultural Research Service, St. Louis, MO 63132, USA
*
Authors to whom correspondence should be addressed.
Current address: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Pozuelo de Alarcón, 28223 Madrid, Spain.
These authors contributed equally to this work.
Metabolites 2020, 10(1), 18; https://doi.org/10.3390/metabo10010018
Received: 6 December 2019 / Revised: 21 December 2019 / Accepted: 21 December 2019 / Published: 28 December 2019
(This article belongs to the Special Issue Metabolomic and Flux Analysis in Plants)
Protein and oil levels measured at maturity are inversely correlated across soybean lines; however, carbon is in limited supply during maturation resulting in tradeoffs for the production of other reserves including oligosaccharides. During the late stages of seed development, the allocation of carbon for storage reserves changes. Lipid and protein levels decline while concentrations of indigestible raffinose family oligosaccharides (RFOs) increase, leading to a decreased crop value. Since the maternal source of carbon is diminished during seed maturation stages of development, carbon supplied to RFO synthesis likely comes from an internal, turned-over source and may contribute to the reduction in protein and lipid content in mature seeds. In this study, fast neutron (FN) mutagenized soybean populations with deletions in central carbon metabolic genes were examined for trends in oil, protein, sugar, and RFO accumulation leading to an altered final composition. Two lines with concurrent increases in oil and protein, by combined 10%, were identified. A delayed switch in carbon allocation towards RFO biosynthesis resulted in extended lipid accumulation and without compromising protein. Strategies for future soybean improvement using FN resources are described. View Full-Text
Keywords: soybeans; fast neutron mutants; protein; oil; raffinose family oligosaccharides; correlations; linear models; central carbon metabolism; seed development soybeans; fast neutron mutants; protein; oil; raffinose family oligosaccharides; correlations; linear models; central carbon metabolism; seed development
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Kambhampati, S.; Aznar-Moreno, J.A.; Hostetler, C.; Caso, T.; Bailey, S.R.; Hubbard, A.H.; Durrett, T.P.; Allen, D.K. On the Inverse Correlation of Protein and Oil: Examining the Effects of Altered Central Carbon Metabolism on Seed Composition Using Soybean Fast Neutron Mutants. Metabolites 2020, 10, 18.

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