Previous Article in Journal
Computer-Aided Design of Peptidomimetic Inhibitors of Falcipain-3: QSAR and Pharmacophore Models

This is an early access version, the complete PDF, HTML, and XML versions will be available soon.

Article

Antiaging Properties of the Ethanol Fractions of Clove (Syzygium aromaticum L.) Bud and Leaf at the Cellular Levels: Study in Yeast Schizosaccharomyces pombe

1
Department of Biology, Dramaga Campus, IPB University, Bogor 16680, Indonesia
2
Department of Biochemistry, Dramaga Campus, IPB University, Bogor 16680, Indonesia
3
Tropical Biopharmaca Research Center, Taman Kencana Campus, IPB University, Bogor 16128, Indonesia
*
Author to whom correspondence should be addressed.
Academic Editor: Helen D. Skaltsa
Sci. Pharm. 2021, 89(4), 45; https://doi.org/10.3390/scipharm89040045
Received: 12 September 2021 / Revised: 22 September 2021 / Accepted: 29 September 2021 / Published: 7 October 2021
The exposure of reactive oxygen species is one of the aging triggers at cellular level. The antioxidants have been used as strategic efforts in overcoming the accumulation of ROS. Previous research using crude extracts of clove bud and leaves showed its potential as an antioxidant agent. However, no data were available regarding the antioxidant and antiaging activities of subsequent fractions of clove extracts. Therefore, this study aimed to analyze the antioxidant and antiaging activities of the n-hexane and ethanol fractions from clove bud and leaves. Antioxidant and antiaging activities were tested at the cellular level using the yeast model Schizosaccharomyces pombe. The highest flavonoid content was shown by clove leaf n-hexane fraction (25.6 mgQE.g−1). However, ethanol fraction of clove bud (FEB) showed the highest antioxidant activity based on TBA and antiglycation assays. FEB (8 μg.mL−1) and leaf ethanol fraction (FEL) (10 μg.mL−1) were able to induce yeast tolerance against oxidative stress. In addition, FEB could induce mitochondrial activity and delay the G1 phase of the cell cycle. FEB was found to be rich in gallic acid and (15Z)-9,12,13-trihydroxy-15-octadecenoic. Based on the data, FEB shows the potential antiaging activity, which is promising for further development as biopharmaceutical product formulations.
Keywords: antioxidant; aging; clove; chronological life span; cell cycle; mitochondria; Schizosaccharomyces pombe; gallic acid; phenolic compounds antioxidant; aging; clove; chronological life span; cell cycle; mitochondria; Schizosaccharomyces pombe; gallic acid; phenolic compounds
MDPI and ACS Style

Lesmana, D.; Andrianto, D.; Astuti, R.I. Antiaging Properties of the Ethanol Fractions of Clove (Syzygium aromaticum L.) Bud and Leaf at the Cellular Levels: Study in Yeast Schizosaccharomyces pombe. Sci. Pharm. 2021, 89, 45. https://doi.org/10.3390/scipharm89040045

AMA Style

Lesmana D, Andrianto D, Astuti RI. Antiaging Properties of the Ethanol Fractions of Clove (Syzygium aromaticum L.) Bud and Leaf at the Cellular Levels: Study in Yeast Schizosaccharomyces pombe. Scientia Pharmaceutica. 2021; 89(4):45. https://doi.org/10.3390/scipharm89040045

Chicago/Turabian Style

Lesmana, Dedy, Dimas Andrianto, and Rika I. Astuti 2021. "Antiaging Properties of the Ethanol Fractions of Clove (Syzygium aromaticum L.) Bud and Leaf at the Cellular Levels: Study in Yeast Schizosaccharomyces pombe" Scientia Pharmaceutica 89, no. 4: 45. https://doi.org/10.3390/scipharm89040045

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop