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Open AccessArticle

Skin Improvement Effects of Gardeniae fructus Extract in HaCaT Keratinocytes, B16F10 Melanocytes, and CCD-986sk Fibroblast Cells

1
Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, Kyungheedae-ro 26, Dongdaemun-gu, Seoul 02447, Korea
2
Department of Korean Medicine, Graduate School, Kyung Hee University, Kyungheedae-ro 26, Dongdaemun-gu, Seoul 02447, Korea
3
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Kyungheedae-ro 26, Dongdaemun-gu, Seoul 02447, Korea
*
Author to whom correspondence should be addressed.
Cosmetics 2019, 6(3), 48; https://doi.org/10.3390/cosmetics6030048
Received: 1 July 2019 / Revised: 25 July 2019 / Accepted: 30 July 2019 / Published: 8 August 2019
(This article belongs to the Special Issue Anti-aging Properties of Natural Compounds)
The development of functional cosmetics with skin improvement effects from natural sources is necessary. In this study, the antioxidant, antiwrinkling, moisturizing, and whitening effects of Gardeniae fructus extract (GF) were investigated in keratinocytes, melanocytes, and fibroblast cells. Antioxidant activity was determined by a DPPH free radical scavenging assay. MMP-1, MMP-9, HAS1, and filaggrin mRNA levels were measured by RT-PCR in keratinocytes and fibroblast cells. MITF and tyrosinase protein levels were evaluated by blotting analysis in melanocytes. DPPH free radical activity was investigated to determine whether GF showed dose-dependent inhibitory activity. GF induced the upregulation of HAS1 and filaggrin mRNA expression in keratinocytes and fibroblast cells. GF led to the downregulation of MMP mRNA levels in keratinocytes and fibroblast cells. Western blotting was performed to confirm the whitening-related protein (MITF and tyrosinase) levels induced by GF in melanocytes, and the inhibitory activity was superior to that of the α-MSH used for the comparison test. GF showed marked antioxidant, antiwrinkling, skin moisturizing, and whitening activity in keratinocytes, melanocytes, and fibroblast cells. Through the results of these experiments, the applicability of GF as a natural and functional cosmetic material was verified. View Full-Text
Keywords: antiwrinkle; moisturizing; whitening; functional cosmetic; natural cosmetic; skin improvement effects antiwrinkle; moisturizing; whitening; functional cosmetic; natural cosmetic; skin improvement effects
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Hong, S.H.; Ku, J.M.; Lee, S.H.; Shim, H.J.; Park, D.S.; Sung, J.W.; Shin, Y.C.; Ko, S.-G. Skin Improvement Effects of Gardeniae fructus Extract in HaCaT Keratinocytes, B16F10 Melanocytes, and CCD-986sk Fibroblast Cells. Cosmetics 2019, 6, 48.

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