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Open AccessArticle

Vitachelox: Protection of the Skin Against Blue Light-Induced Protein Carbonylation

1
Indena S.p.A, 20139 Milan, Italy
2
OxiProteomics, 75005 Paris, France
3
Polistudium SRL, 20135 Milan, Italy
*
Author to whom correspondence should be addressed.
Cosmetics 2019, 6(3), 49; https://doi.org/10.3390/cosmetics6030049
Received: 17 July 2019 / Revised: 5 August 2019 / Accepted: 7 August 2019 / Published: 8 August 2019
Protein carbonylation (PC) is a marker of reactive oxygen species-mediated alterations induced by external stimuli such as UV and blue light irradiation. In this study, we investigated the protective effect of Vitachelox®, a mixture of three standardized natural extracts rich in polyphenols, against PC induced by blue light irradiation in human keratinocytes. We tested eight experimental conditions, including Vitachelox® 0.01% and 0.005% w/v, used for 6 or 24 h before irradiation, and a solution of N-acetylcysteine (NAC) as positive control of protection. PC was evaluated by fluorescence microscopy in situ and by absolute quantification (Carbonyl Score) upon protein extraction and separation. Both the in situ visualization study and the carbonyl score showed a considerable increase in protein oxidative damage upon blue light irradiation, and a decrease in PC in the presence of Vitachelox®. In particular, Vitachelox® 0.005% showed superior results compared to NAC in terms of carbonyl score and protein quality, and it was estimated to exert a protective action against blue-light irradiation ranging from 72% (24 h) to 82% (6 h). The protective antioxidant effect of Vitachelox®, together with the anti-inflammatory and anti-microbial properties previously reported, make this natural active ingredient a valuable tool in the maintenance of healthy skin. View Full-Text
Keywords: Vitachelox®; protein carbonylation; blue light; anti-oxidant; human keratinocytes; natural extracts; polyphenols Vitachelox®; protein carbonylation; blue light; anti-oxidant; human keratinocytes; natural extracts; polyphenols
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Togni, S.; Maramaldi, G.; Cavagnino, A.; Corti, A.; Giacomelli, L. Vitachelox: Protection of the Skin Against Blue Light-Induced Protein Carbonylation. Cosmetics 2019, 6, 49.

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