Next Article in Journal
Anti-aging Effects of Select Botanicals: Scientific Evidence and Current Trends
Next Article in Special Issue
Nanoemulsions as a Form of Perfumery Products
Previous Article in Journal
Capacitive Imaging for Skin Characterizations and Solvent Penetration Measurements
Open AccessArticle

Safety Assessment of Nano-Hydroxyapatite as an Oral Care Ingredient according to the EU Cosmetics Regulation

1
Cell Therapy and Tissue Engineering Group, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands, 07122 Palma, Spain
2
Department of Fundamental Biology and Health Sciences, University of the Balearic Islands, 07122 Palma, Spain
3
i3S–Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal
4
INEB—Instituto de Engenharia Biomédica, Universidade do Porto, 4200-135 Porto, Portugal
5
Faculdade de Engenharia da Universidade do Porto, Universidade do Porto, 4200-465 Porto, Portugal
6
FLUIDINOVA, S.A., 4470-605 Maia, Portugal
7
Balearic Islands Health Research Institute (IdISBa), 07010 Palma, Spain
*
Authors to whom correspondence should be addressed.
Cosmetics 2018, 5(3), 53; https://doi.org/10.3390/cosmetics5030053
Received: 26 July 2018 / Revised: 5 September 2018 / Accepted: 6 September 2018 / Published: 11 September 2018
Hydroxyapatite nanoparticles (HAP-NP) are incorporated in oral care products such as toothpastes and mouthwashes to treat dental sensitivity or to promote enamel remineralisation. Despite the good performance of HAP-NP in this application, it is important to ensure its safety for consumers. For that reason, the Scientific Committee on Consumer Safety (SCCS) evaluated the safety of HAP-NP as an oral care ingredient, but the issued opinion was not completely conclusive and the SCCS recommended that additional tests should be performed. Here, we used a commercially available human gingival epithelium (HGE) as a non-animal alternative and MTT cell viability, LDH activity, and IL-1alpha production were evaluated after 3.1% HAP-NP treatment for 10 min, 1 h, and 3 h. Moreover, the absorption of HAP-NP in the gingival tissue was assessed by transmission electron microscopy (TEM) analysis. Finally, the dissolution behaviour of HAP-NP in simulated gastric fluid was also investigated. No deleterious effect was observed for HGE tissues incubated with HAP-NP for all time-points and parameters evaluated. Moreover, a complete dissolution of 3.1% HAP-NP in simulated gastric fluid was observed after 7.5 min at 37 °C. In conclusion, our results evidence the safety of HAP-NP for oral care products with the use of an in vitro replacement alternative for human gingival epithelium and a simulated gastric fluid assay. View Full-Text
Keywords: hydroxyapatite nanoparticles; human gingival epithelium; oral care; EU cosmetics regulation; safety evaluation; in vitro biocompatibility; dissolution in simulated gastric fluid hydroxyapatite nanoparticles; human gingival epithelium; oral care; EU cosmetics regulation; safety evaluation; in vitro biocompatibility; dissolution in simulated gastric fluid
Show Figures

Figure 1

MDPI and ACS Style

Ramis, J.M.; Coelho, C.C.; Córdoba, A.; Quadros, P.A.; Monjo, M. Safety Assessment of Nano-Hydroxyapatite as an Oral Care Ingredient according to the EU Cosmetics Regulation. Cosmetics 2018, 5, 53. https://doi.org/10.3390/cosmetics5030053

AMA Style

Ramis JM, Coelho CC, Córdoba A, Quadros PA, Monjo M. Safety Assessment of Nano-Hydroxyapatite as an Oral Care Ingredient according to the EU Cosmetics Regulation. Cosmetics. 2018; 5(3):53. https://doi.org/10.3390/cosmetics5030053

Chicago/Turabian Style

Ramis, Joana M.; Coelho, Catarina C.; Córdoba, Alba; Quadros, Paulo A.; Monjo, Marta. 2018. "Safety Assessment of Nano-Hydroxyapatite as an Oral Care Ingredient according to the EU Cosmetics Regulation" Cosmetics 5, no. 3: 53. https://doi.org/10.3390/cosmetics5030053

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop