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Cosmetics 2016, 3(2), 15;

In Vitro DVS Approach to Evaluate Skin Reparation

IQAC-CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain
Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via F. Marzolo 5, 35231 Padova, Italy
Unifarco S.p.A., Via Cal Longa 62, 32035 Santa Giustina (BL), Italy
Author to whom correspondence should be addressed.
Academic Editor: Marie Lodén
Received: 10 December 2015 / Revised: 17 March 2016 / Accepted: 18 March 2016 / Published: 31 March 2016
(This article belongs to the Special Issue Efficacy Assessment of Cosmetics)
Full-Text   |   PDF [12685 KB, uploaded 31 March 2016]   |  


The stratum corneum (SC), the most superficial layer of the skin, is directly responsible for the skin’s barrier function. The intercellular lipids in the SC play an important role in the regulation of the skin’s water-holding capacity. The modification of the intercellular lipid organization and composition may impair these properties. The aim of the present study is to describe a new in vitro approach of the repairing capacity evaluation of lipid formulations on skin with the use of absorption and desorption curves. The formulations were applied on lipid-extracted SC, and the possible SC reparation was assessed with the use of a thermogravimetric balance (DVS). Moisture absorption/desorption experiments proved to be adequate for the evaluation of the repairing capacity of formulations applied on damaged skin. Besides, freeze-substitution transmission electron microscopy (FSTEM) images showed some lipid bilayers, indicating a lipid bilayer reconstitution due to the applied formulations. View Full-Text
Keywords: stratum corneum; lipids; thermogravimetric analysis; permeability; water diffusion stratum corneum; lipids; thermogravimetric analysis; permeability; water diffusion

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Barba, C.; Alonso, C.; Semenzato, A.; Baratto, G.; Coderch, L. In Vitro DVS Approach to Evaluate Skin Reparation. Cosmetics 2016, 3, 15.

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