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Open AccessArticle

N-acetyl-L-cysteine Prevents Lactate-Mediated PGC1-alpha Expression in C2C12 Myotubes

Graduate School of Medicine, Kyoto University, Kyoto 606-8315, Japan
Research Institute of Sport Science, University of Physical Education, 1525 Budapest, Hungary
Graduate School of Sports and Health Science, Doshisha University, Kyotanabe 610-0394, Japan
Author to whom correspondence should be addressed.
Biology 2019, 8(2), 44;
Received: 21 May 2019 / Revised: 4 June 2019 / Accepted: 5 June 2019 / Published: 10 June 2019
(This article belongs to the Section Cell Biology)
PDF [1330 KB, uploaded 10 June 2019]


Background: Exercise induces many physiological adaptations. Recently, it has been proposed that some of these adaptations are induced by exercise-mediated lactate production. In this study, we aimed to investigate in vitro the effect of lactate in cultured myotubes and whether antioxidants could inhibit the effect. Methods: Differentiated myotubes were cultured at different concentrations of L-lactate (0, 10, 30, 50 mM) in the absence or presence of an antioxidant, N-acetyl-L-cysteine (Nac). The temporal effect of lactate exposure in myotubes was also explored. Results: Two hours of exposure to 50 mM L-lactate and six hours of exposure to 30 or 50 mM L-lactate caused a significant increase in PGC1-alpha (peroxisome proliferator-activated receptor γ coactivator-1α) expression in the myotubes. This up-regulation was suppressed by 2 mM Nac. Intermittent and continuous lactate exposure caused similar PGC1-alpha up-regulation. These results suggest that the increase in PGC1-alpha expression is mediated by reactive oxygen species (ROS) production from lactate metabolism and that both continuous and intermittent exposure to L-lactate can cause the up-regulation. View Full-Text
Keywords: lactate signaling; ROS; PGC1-alpha; myotubes lactate signaling; ROS; PGC1-alpha; myotubes

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Nalbandian, M.; Radak, Z.; Takeda, M. N-acetyl-L-cysteine Prevents Lactate-Mediated PGC1-alpha Expression in C2C12 Myotubes. Biology 2019, 8, 44.

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