Next Article in Journal
Hot Corrosion Behavior of BaLa2Ti3O10 Thermal Barrier Ceramics in V2O5 and Na2SO4 + V2O5 Molten Salts
Next Article in Special Issue
Surface Functionalization of Bioactive Glasses with Polyphenols from Padina pavonica Algae and In Situ Reduction of Silver Ions: Physico-Chemical Characterization and Biological Response
Previous Article in Journal
Novel Preparation of Noncovalent Modified GO Using RAFT Polymerization to Reinforce the Performance of Waterborne Epoxy Coatings
Previous Article in Special Issue
Functionalization of Ceramic Coatings for Enhancing Integration in Osteoporotic Bone: A Systematic Review
Open AccessFeature PaperArticle

Biomimetic Properties of Force-Spun PHBV Membranes Functionalised with Collagen as Substrates for Biomedical Application

School of Engineering, Newcastle University, Newcastle-Upon-Tyne NE1 7RU, UK
Nonwovens Research, School of Design, University of Leeds, Leeds LS2 9JT, UK
Author to whom correspondence should be addressed.
Coatings 2019, 9(6), 350;
Received: 3 May 2019 / Revised: 22 May 2019 / Accepted: 24 May 2019 / Published: 28 May 2019
(This article belongs to the Special Issue Surfaces Modification and Analysis for Innovative Biomaterials)
The force-spinning process parameters (i.e., spin speed, spinneret-collector distance, and polymer concentration), optimised and characterised in previous work by this group, allowed the rapid fabrication of large quantities of high surface area poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) polymeric fibre membranes. This paper examined the potential application for force-spun PHBV fibres functionalised with type I collagen for tissue regeneration applications. PHBV fibre scaffolds provide a biologically suitable substrate to guide the regeneration of dermal tissues, however, have poor cellular adhesion properties. The grafting of collagen type-I to PHBV fibres demonstrated improved cell adhesion and growth in Neo-NHDF (neonatal human dermal fibroblasts) fibroblasts. The examination of fibre morphology, thermal properties, collagen content, and degradability was used to contrast the physicochemical properties of the PHBV and PHBV-Collagen fibres. Biodegradation models using phosphate buffered saline determined there was no appreciable change in mass over the course of 6 weeks; a Sirius Red assay was performed on degraded samples, showing no change in the quantity of collagen. Cell metabolism studies showed an increase in cell metabolism on conjugated samples after three and 7 days. In addition, in vitro cytocompatibility studies demonstrated superior cell activity and adhesion on conjugated samples over 7 days. View Full-Text
Keywords: PHBV; collagen; force spinning; functionalization; tissue engineering PHBV; collagen; force spinning; functionalization; tissue engineering
Show Figures

Figure 1

MDPI and ACS Style

McColgan-Bannon, K.I.S.; Upson, S.; Gentile, P.; Tausif, M.; Russell, S.; Dalgarno, K.; Ferreira, A.M. Biomimetic Properties of Force-Spun PHBV Membranes Functionalised with Collagen as Substrates for Biomedical Application. Coatings 2019, 9, 350.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop