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Films of Chitosan and Aloe vera for Maintaining the Viability and Antifungal Activity of Lactobacillus paracasei TEP6

Instituto de Biociencias, Universidad Autónoma de Chiapas, Boulevard Príncipe Akishino sin número Colonia Solidaridad 2000, Tapachula CP 30798, Mexico
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Coatings 2020, 10(3), 259; https://doi.org/10.3390/coatings10030259
Received: 31 January 2020 / Revised: 3 March 2020 / Accepted: 5 March 2020 / Published: 10 March 2020
(This article belongs to the Special Issue Biodegradable Films and Composite Coatings: Current and Future Trends)
The present study aimed to evaluate the effect of Aloe vera addition on the viability and antifungal activity of TEP6 (Lactobacillus paracasei) bacteria immobilized on chitosan films for 28 days. Different chitosan and A. vera proportions and carbon sources at several pH values were tested as formulations for supporting the microorganism. Bacterial viability was maintained in freshly made films, with values of 10.4, 10.8 and 10.9 log CFU·g−1 for the formulations containing 70% (T11), 100% (T8) and 100% (T16) of A. vera, respectively. The same formulations (T8, T11 and T16) maintained bacterial viability for 14 days of film storage with a loss to values of 9.5 log CFU·g−1. By applying a quarter fraction 25–2 experimental design with an array of five factors, the factors with the greatest influence on viability and antifungal activity were determined. The optimal conditions for viability were the formulation with 100% A. vera, pH 4.5 and 0.1 M glucose. The antifungal activity of fresh films was influenced by the formulation with 10 g·L−1 glycerol and 100% A. vera, showing a 60% inhibition of fungal (Colletotrichum gloeosporioides) growth. The films developed in this study may have the potential to be used as coatings on vegetal products susceptible to attack by Colletotrichum gloesporioides. View Full-Text
Keywords: Lactobacillus paracasei; Colletotrichum gloeosporioides; MTT; glycerol; inhibition Lactobacillus paracasei; Colletotrichum gloeosporioides; MTT; glycerol; inhibition
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Barragán-Menéndez, C.; Gálvez-López, D.; Rosas-Quijano, R.; Salvador-Figueroa, M.; Ovando-Medina, I.; Vázquez-Ovando, A. Films of Chitosan and Aloe vera for Maintaining the Viability and Antifungal Activity of Lactobacillus paracasei TEP6. Coatings 2020, 10, 259.

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