Activity of Temporin A and Short Lipopeptides Combined with Gentamicin against Biofilm Formed by Staphylococcus aureus and Pseudomonas aeruginosa
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains and Culture Conditions
4.2. Antimicrobials
4.3. Minimum Inhibitory Concentration (MIC)
4.4. Fractional Inhibitory Concentration (FIC)
4.5. Minimum Biofilm Eradication Concentration (MBEC)
4.6. Activity of Gentamicin Applied in Combination with AMPs against Biofilms Formed by SA and PA on 96-well PS
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Compound | SA | PA | ||||
---|---|---|---|---|---|---|
MIC | ΣFIC with Gentamicin | Interaction | MIC | ΣFIC with Gentamicin | Interaction | |
Gentamicin | 0.25 | – | – | 0.5 | – | – |
Temporin A | 8 | 1 | indifferent | 512 | 1 | indifferent |
Lipopeptide 1 | 8 | 2 | indifferent | 16 | 2 | indifferent |
Lipopeptide 2 | 16 | 1 | indifferent | 32 | 2 | indifferent |
Compound | MBEC (mg/L) | |||||
---|---|---|---|---|---|---|
SA | PA | |||||
MBEC90 | MBEC50 | Enhancement of Activity of Gentamicin | MBEC90 | MBEC50 | Enhancement of Activity of Gentamicin | |
Gentamicin | >32 | 1 | – | 2 | 2 | – |
Temporin A | 64 | 32 | +++ | >512 | >512 | ++ |
Lipopeptide 1 | 32 | 32 | +++ | 256 | 128 | + |
Lipopeptide 2 | >64 | >64 | +++ | >512 | >512 | + |
Compound | MBEC 90 (mg/L) | |||
---|---|---|---|---|
SA | PA | |||
Gentamicin | Peptide | Gentamicin | Peptide | |
Gentamicin alone | >32 | – | 2 | – |
Lipopeptide 1 alone | – | 32 | – | 256 |
Gentamicin + L-1 | 1 | 4 | 1 | 128 |
Lipopeptide 2 alone | – | >64 | – | >512 |
Gentamicin + L-2 | 1 | 32 | 1 | 512 |
Temporin A alone | – | 64 | – | >512 |
Gentamycin + TA | 0.125 | 32 | 0.5 | 512 |
Compound | MBEC 50 (mg/L) | |||
---|---|---|---|---|
SA | PA | |||
Gentamicin | Peptide | Gentamicin | Peptide | |
Gentamicin alone | 1 | – | 2 | – |
Lipopeptide 1 alone | – | 32 | – | 128 |
Gentamicin + L-1 | 0.5 | 8 | 1 | 64 |
Lipopeptide 2 alone | – | > 64 | – | > 512 |
Gentamicin + L-2 | 0.5 | 64 | 1 | 256 |
Temporin A alone | – | 32 | – | > 512 |
Gentamicin + TA | 0.125 | 8 | 0.5 | 256 |
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Paduszynska, M.A.; Greber, K.E.; Paduszynski, W.; Sawicki, W.; Kamysz, W. Activity of Temporin A and Short Lipopeptides Combined with Gentamicin against Biofilm Formed by Staphylococcus aureus and Pseudomonas aeruginosa. Antibiotics 2020, 9, 566. https://doi.org/10.3390/antibiotics9090566
Paduszynska MA, Greber KE, Paduszynski W, Sawicki W, Kamysz W. Activity of Temporin A and Short Lipopeptides Combined with Gentamicin against Biofilm Formed by Staphylococcus aureus and Pseudomonas aeruginosa. Antibiotics. 2020; 9(9):566. https://doi.org/10.3390/antibiotics9090566
Chicago/Turabian StylePaduszynska, Malgorzata Anna, Katarzyna Ewa Greber, Wojciech Paduszynski, Wieslaw Sawicki, and Wojciech Kamysz. 2020. "Activity of Temporin A and Short Lipopeptides Combined with Gentamicin against Biofilm Formed by Staphylococcus aureus and Pseudomonas aeruginosa" Antibiotics 9, no. 9: 566. https://doi.org/10.3390/antibiotics9090566