Next Article in Journal
Secondary Antibiotic Resistance, Correlation between Genotypic and Phenotypic Methods and Treatment in Helicobacter pylori Infected Patients: A Retrospective Study
Next Article in Special Issue
Mycogenic Metal Nanoparticles for the Treatment of Mycobacterioses
Previous Article in Journal
Antibiotic Tolerance of Staphylococcus aureus Biofilm in Periprosthetic Joint Infections and Antibiofilm Strategies
Previous Article in Special Issue
Active Pulmonary Tuberculosis in Elderly Patients: A 2016–2019 Retrospective Analysis from an Italian Referral Hospital
Article

Genetic Identification and Drug-Resistance Characterization of Mycobacterium tuberculosis Using a Portable Sequencing Device. A Pilot Study

1
Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, El Paso, TX 79905, USA
2
Servicio de Micobacterias, Instituto Nacional de Enfermedades Infecciosas (INEI)-ANLIS and CONICET, Buenos Aires C1282AFF, Argentina
3
The Jackson Laboratory for Genomic Medicine, Farmington, CT 06032, USA
*
Author to whom correspondence should be addressed.
Antibiotics 2020, 9(9), 548; https://doi.org/10.3390/antibiotics9090548
Received: 29 June 2020 / Revised: 19 August 2020 / Accepted: 26 August 2020 / Published: 27 August 2020
(This article belongs to the Special Issue Mycobacterial Infections and Therapy)
Clinical management of tuberculosis (TB) in endemic areas is often challenged by a lack of resources including laboratories for Mycobacterium tuberculosis (Mtb) culture. Traditional phenotypic drug susceptibility testing for Mtb is costly and time consuming, while PCR-based methods are limited to selected target loci. We herein utilized a portable, USB-powered, long-read sequencing instrument (MinION), to investigate Mtb genomic DNA from clinical isolates to determine the presence of anti-TB drug-resistance conferring mutations. Data analysis platform EPI2ME and antibiotic-resistance analysis using the real time ARMA workflow, identified Mtb species as well as extensive resistance gene profiles. The approach was highly sensitive, being able to detect almost all described drug resistance conferring mutations based on previous whole genome sequencing analysis. Our findings are supportive of the practical use of this system as a suitable method for the detection of antimicrobial resistance genes, and effective in providing Mtb genomic information. Future improvements in the error rate through statistical analysis, drug resistance prediction algorithms and reference databases would make this a platform suited for the clinical setting. The small size, relatively inexpensive cost of the device, as well as its rapid and simple library preparation protocol and analysis, make it an attractive option for settings with limited laboratory infrastructure. View Full-Text
Keywords: next-generation sequencing; MinION; tuberculosis; drug resistance next-generation sequencing; MinION; tuberculosis; drug resistance
MDPI and ACS Style

Cervantes, J.; Yokobori, N.; Hong, B.-Y. Genetic Identification and Drug-Resistance Characterization of Mycobacterium tuberculosis Using a Portable Sequencing Device. A Pilot Study. Antibiotics 2020, 9, 548. https://doi.org/10.3390/antibiotics9090548

AMA Style

Cervantes J, Yokobori N, Hong B-Y. Genetic Identification and Drug-Resistance Characterization of Mycobacterium tuberculosis Using a Portable Sequencing Device. A Pilot Study. Antibiotics. 2020; 9(9):548. https://doi.org/10.3390/antibiotics9090548

Chicago/Turabian Style

Cervantes, Jorge, Noemí Yokobori, and Bo-Young Hong. 2020. "Genetic Identification and Drug-Resistance Characterization of Mycobacterium tuberculosis Using a Portable Sequencing Device. A Pilot Study" Antibiotics 9, no. 9: 548. https://doi.org/10.3390/antibiotics9090548

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop