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Article

New Insights into Bioactive Compounds from the Medicinal Plant Spathodea campanulata P. Beauv. and Their Activity against Helicobacter pylori

1
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy
2
Department of Biochemistry, Faculty of Science, The University of Yaounde I, P. Box 812 Yaounde, Cameroon
3
Doctorate School in Clinical and Experimental Medicine (CEM), University of Modena and Reggio Emilia, 41125 Modena, Italy
4
Department of Pharmacy and Biotechnology, Alma Mater Studiorum University of Bologna, Viale Fanin 44, 40127 Bologna, Italy
5
Nigerian Institute of Medical Research, PMB 2013, Yaba, Lagos 100001, Nigeria
*
Authors to whom correspondence should be addressed.
Antibiotics 2020, 9(5), 258; https://doi.org/10.3390/antibiotics9050258
Received: 21 March 2020 / Revised: 12 May 2020 / Accepted: 13 May 2020 / Published: 15 May 2020
(This article belongs to the Special Issue Novel Targets and Mechanisms in Antimicrobial Drug Discovery)
The medicinal plant Spathodea campanulata P. Beauv. (Bignoniaceae) has been traditionally applied for the prevention and treatment of diseases of the kidney and urinary system, the skin, the gastrointestinal tract, and inflammation in general. The present work shows for the first time how chemical components from this plant inhibit Helicobacter pylori growth by urease inhibition and modulation of virulence factors. The crude extract and the main fractions of S. campanulata bark were tested on H. pylori isolated strains and the active ones were further fractionated. Fractions and sub-fractions of the plant crude extract were characterized by ultra-high-performance liquid chromatographic tandem high resolution-mass spectrometry detection (UHPLC-HRMS). Several phenolics and triterpenoids were identified. Among the sub-fractions obtained, SB2 showed the capacity to inhibit H. pylori urease in a heterologous bacterial model. One additional sub-fraction (SE3) was able to simultaneously modulate the expression of two adhesins (HopZ and BabA) and one cytotoxin (CagA). The flavonol kaempferol was identified as the most interesting compound that deserves further investigation as a new hit for its capacity to modulate H. pylori virulence factors. View Full-Text
Keywords: Spathodea campanulata; phenolics; UHPLC-MS; Helicobacter pylori; urease; adhesins; cytotoxins Spathodea campanulata; phenolics; UHPLC-MS; Helicobacter pylori; urease; adhesins; cytotoxins
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MDPI and ACS Style

Ngnameko, C.R.; Marchetti, L.; Zambelli, B.; Quotadamo, A.; Roncarati, D.; Bertelli, D.; Njayou, F.N.; Smith, S.I.; Moundipa, P.F.; Costi, M.P.; Pellati, F. New Insights into Bioactive Compounds from the Medicinal Plant Spathodea campanulata P. Beauv. and Their Activity against Helicobacter pylori. Antibiotics 2020, 9, 258. https://doi.org/10.3390/antibiotics9050258

AMA Style

Ngnameko CR, Marchetti L, Zambelli B, Quotadamo A, Roncarati D, Bertelli D, Njayou FN, Smith SI, Moundipa PF, Costi MP, Pellati F. New Insights into Bioactive Compounds from the Medicinal Plant Spathodea campanulata P. Beauv. and Their Activity against Helicobacter pylori. Antibiotics. 2020; 9(5):258. https://doi.org/10.3390/antibiotics9050258

Chicago/Turabian Style

Ngnameko, Corinne R., Lucia Marchetti, Barbara Zambelli, Antonio Quotadamo, Davide Roncarati, Davide Bertelli, Frederic N. Njayou, Stella I. Smith, Paul F. Moundipa, Maria P. Costi, and Federica Pellati. 2020. "New Insights into Bioactive Compounds from the Medicinal Plant Spathodea campanulata P. Beauv. and Their Activity against Helicobacter pylori" Antibiotics 9, no. 5: 258. https://doi.org/10.3390/antibiotics9050258

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