Next Article in Journal
Antimicrobial Resistance, Pharmacists, and Appreciative Inquiry: Development of a Novel Measurement Tool
Previous Article in Journal
High Prevalence of blaCTX-M-15 Gene among Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates Causing Extraintestinal Infections in Bangladesh
Open AccessArticle

Towards the Identification of Antibiotic-Resistant Bacteria Causing Urinary Tract Infections Using Volatile Organic Compounds Analysis—A Pilot Study

1
Department of Applied Sciences, Faculty of Health and Applied Sciences, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK
2
Department of Chemistry and Biodynamics of Food, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, 10-748 Olsztyn, Poland
3
Applied Statistics Group, Department of Engineering, Design and Mathematics, Faculty of Environment and Technology, University of the West of England, Bristol BS16 1QY, UK
4
School of Cellular & Molecular Medicine, Faculty of Life Sciences, University of Bristol, Bristol BS8 1TD, UK
5
Bristol Royal Infirmary and Bristol Urological Institute, Southmead Hospital, Bristol BS10 5BN, UK
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Antibiotics 2020, 9(11), 797; https://doi.org/10.3390/antibiotics9110797
Received: 28 September 2020 / Revised: 8 November 2020 / Accepted: 10 November 2020 / Published: 11 November 2020
(This article belongs to the Special Issue Metabolic Profiling for Microbial Resistance)
Antibiotic resistance is an unprecedented threat to modern medicine. The analysis of volatile organic compounds (VOCs) from bacteria potentially offers a rapid way to determine antibiotic susceptibility in bacteria. This study aimed to find the optimal conditions to obtain the maximum number of VOCs detected which next allowed the assessment of differences in VOC profiles between susceptible and resistant isolates of Escherichia coli causing urinary tract infections. The analysis of VOCs in the headspace above the bacterial cultures allowed the distinguishing of resistant and susceptible bacteria based on the abundance of six VOCs with 85.7% overall accuracy. The results of this preliminary study are promising, and with development could lead to a practical, faster diagnostic method for use in routine microbiology. View Full-Text
Keywords: volatile; metabolite; profiles; gas chromatography-mass spectrometry; antibiotic resistance; bacteria; urinary tract infection; susceptibility; E. coli volatile; metabolite; profiles; gas chromatography-mass spectrometry; antibiotic resistance; bacteria; urinary tract infection; susceptibility; E. coli
Show Figures

Figure 1

MDPI and ACS Style

Hewett, K.; Drabińska, N.; White, P.; Avison, M.B.; Persad, R.; Ratcliffe, N.; Costello, B.L. Towards the Identification of Antibiotic-Resistant Bacteria Causing Urinary Tract Infections Using Volatile Organic Compounds Analysis—A Pilot Study. Antibiotics 2020, 9, 797.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop