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Article

A Pilot Study of the Synergy between Two Antimicrobial Peptides and Two Common Antibiotics

1
School of Life Science, Engineering & Design, Saxion University of Applied Science, 7513 AB Enschede, The Netherlands
2
School of Optometry and Vision Science, University of New South Wales, Sydney, NSW 2052, Australia
3
Ophthalmic Research Group, Optometry School, Aston University, Birmingham B4 7ET, UK
*
Author to whom correspondence should be addressed.
Antibiotics 2019, 8(2), 60; https://doi.org/10.3390/antibiotics8020060
Received: 26 March 2019 / Revised: 17 April 2019 / Accepted: 5 May 2019 / Published: 9 May 2019
Background: Frequent and unrestricted use of antibiotics has been associated with the development of antibiotic resistance by microorganisms. Thus, there is a need to find novel antibacterial agents or a combination of agents as the first line of treatment for various infections. This study aimed to investigate the synergy between antimicrobial peptide (AMP) combinations or between AMP-antibiotics combinations using two common pathogens, Pseudomonas aeruginosa and Staphylococcus aureus. Methods: The AMPs melimine, Mel4 and protamine, and antibiotics cefepime and ciprofloxacin were used in this study. The minimum inhibitory concentration (MIC) of each were evaluated against P. aeruginosa and S. aureus strains by a microtiter broth dilution. Based on the MIC of each antimicrobial agent, a checkerboard assay was performed to investigate the synergy between them, which was expressed as the fractional inhibitory concentration (FIC). Results: The combination of melimine and ciprofloxacin showed synergistic activity against antibiotic sensitive or resistant strains of P. aeruginosa and with FIC values ≤0.5. Conclusion: Combinations of AMPs and the fluoroquinolone ciprofloxacin is a promising method for reducing resistance to the fluoroquinolone of P. aeruginosa. View Full-Text
Keywords: synergy; antimicrobial peptides; antibiotics; Staphylococcus; Pseudomonas synergy; antimicrobial peptides; antibiotics; Staphylococcus; Pseudomonas
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MDPI and ACS Style

Kampshoff, F.; Willcox, M.D.P.; Dutta, D. A Pilot Study of the Synergy between Two Antimicrobial Peptides and Two Common Antibiotics. Antibiotics 2019, 8, 60. https://doi.org/10.3390/antibiotics8020060

AMA Style

Kampshoff F, Willcox MDP, Dutta D. A Pilot Study of the Synergy between Two Antimicrobial Peptides and Two Common Antibiotics. Antibiotics. 2019; 8(2):60. https://doi.org/10.3390/antibiotics8020060

Chicago/Turabian Style

Kampshoff, Franziska, Mark D. P. Willcox, and Debarun Dutta. 2019. "A Pilot Study of the Synergy between Two Antimicrobial Peptides and Two Common Antibiotics" Antibiotics 8, no. 2: 60. https://doi.org/10.3390/antibiotics8020060

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