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Article

Prophylactic Antibiofilm Activity of Antibiotic-Loaded Bone Cements against Gram-Negative Bacteria

by 1, 1,2,3,4, 1,2,4,5 and 1,2,4,*
1
Centre International de Recherche en Infectiologie, CIRI, Inserm U1111, CNRS UMR5308, ENS de Lyon, UCBL1, 69007 Lyon, France
2
Université Claude Bernard Lyon 1, 69100 Lyon, France
3
Service de Maladies Infectieuses, Hôpital de la Croix-Rousse, Hospices Civils de Lyon, 69002 Lyon, France
4
Centre Interrégional de Référence des Infections Ostéo-Articulaires Complexes (CRIOAc Lyon), Hospices Civils de Lyon, 69002 Lyon, France
5
Laboratoire de Bactériologie, Institut des Agents Infectieux, Hôpital de la Croix-Rousse, Hospices Civils de Lyon, 69002 Lyon, France
*
Author to whom correspondence should be addressed.
Academic Editor: Joerg Overhage
Antibiotics 2022, 11(2), 137; https://doi.org/10.3390/antibiotics11020137
Received: 15 December 2021 / Revised: 13 January 2022 / Accepted: 19 January 2022 / Published: 21 January 2022
(This article belongs to the Special Issue Strategies against Bacterial Biofilm Formation)
Gram-negative bacilli can be responsible for prosthetic joint infection (PJI) even if staphylococci are the main involved pathogens. Gram-negative PJIs (GN-PJI) are considered difficult-to-treat infections due to the increase in antimicrobial resistance and biofilm formation. To minimize the risk of infection in cases of arthroplasties with cemented prosthesis, bone cement can be loaded with antibiotics, especially gentamicin. In this study, we aimed to compare the prophylactic antibiofilm activity of ready-to-use antibiotic-loaded bone cements (ALBC), already commercialized or new prototypes. We compared ALBCs containing gentamicin alone, gentamicin plus vancomycin, gentamicin plus clindamycin, gentamicin plus Fosfomycin, and fosfomycin alone, to plain cement (no antibiotic); these comparisons were conducted to investigate the biofilm formation of three strains of Escherichia coli, three strains of Pseudomonas aeruginosa and two strains of Klebsiella pneumoniae, with or without specific resistance to gentamicin or fosfomycin. We reported that ALBC containing gentamicin and clindamycin (COPAL G+C) seems to be the most interesting ALBC of our tested panel for the prevention of biofilm formation by gentamicin-susceptible strains, even if clindamycin is not effective against Gram-negative bacteria. However, gentamicin-resistant strains are still a problem, and further studies are needed to identify an antibiotic to associate with gentamicin for an efficient dual ALBC against Gram-negative bacteria. View Full-Text
Keywords: antibiotic-loaded bone cement; prosthetic joint infection; biofilm; Pseudomonas aeruginosa; Escherichia coli; Klebsiella pneumoniae antibiotic-loaded bone cement; prosthetic joint infection; biofilm; Pseudomonas aeruginosa; Escherichia coli; Klebsiella pneumoniae
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MDPI and ACS Style

Cara, A.; Ferry, T.; Laurent, F.; Josse, J. Prophylactic Antibiofilm Activity of Antibiotic-Loaded Bone Cements against Gram-Negative Bacteria. Antibiotics 2022, 11, 137. https://doi.org/10.3390/antibiotics11020137

AMA Style

Cara A, Ferry T, Laurent F, Josse J. Prophylactic Antibiofilm Activity of Antibiotic-Loaded Bone Cements against Gram-Negative Bacteria. Antibiotics. 2022; 11(2):137. https://doi.org/10.3390/antibiotics11020137

Chicago/Turabian Style

Cara, Andréa, Tristan Ferry, Frédéric Laurent, and Jérôme Josse. 2022. "Prophylactic Antibiofilm Activity of Antibiotic-Loaded Bone Cements against Gram-Negative Bacteria" Antibiotics 11, no. 2: 137. https://doi.org/10.3390/antibiotics11020137

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