Next Article in Journal
Effect of Different Piperacillin-Tazobactam Dosage Regimens on Synergy of the Combination with Tobramycin against Pseudomonas aeruginosa for the Pharmacokinetics of Critically Ill Patients in a Dynamic Infection Model
Next Article in Special Issue
Diagnosis of Bloodstream Infections: An Evolution of Technologies towards Accurate and Rapid Identification and Antibiotic Susceptibility Testing
Previous Article in Journal
Factors Affecting Antibiotic Prescription among Hospital Physicians in a Low-Antimicrobial-Resistance Country: A Qualitative Study
Previous Article in Special Issue
Molecular Detection of Fluoroquinolone Resistance among Multidrug-, Extensively Drug-, and Pan-Drug-Resistant Campylobacter Species in Egypt
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Antibiotics
Volume 11
Issue 1
10.3390/antibiotics11010100
antibiotics-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Communication

A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria—A Pilot Study

by
Jolein Gyonne Elise Laumen
1,2,*,
Saïd Abdellati
3,
Christophe Van Dijck
1,2,
Delphine Martiny
4,5,
Irith De Baetselier
3,
Sheeba Santhini Manoharan-Basil
1,
Dorien Van den Bossche
3 and
Chris Kenyon
1,6
1
STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium
2
Laboratory of Medical Microbiology, University of Antwerp, 2610 Wilrijk, Belgium
3
Clinical and Reference Laboratory, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium
4
Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), Université Libre de Bruxelles, 1000 Brussels, Belgium
5
Faculté de Médecine et Pharmacie, Université de Mons, 7000 Mons, Belgium
6
Division of Infectious Diseases and HIV Medicine, University of Cape Town, Cape Town 7700, South Africa
*
Author to whom correspondence should be addressed.
Antibiotics 2022, 11(1), 100; https://doi.org/10.3390/antibiotics11010100
Submission received: 13 December 2021 / Revised: 7 January 2022 / Accepted: 10 January 2022 / Published: 13 January 2022
(This article belongs to the Special Issue Rapid Diagnostics of the Antimicrobial Resistance)
Review Reports Versions Notes

Abstract

Commensal Neisseria provide a reservoir of resistance genes that can be transferred to the pathogens Neisseria gonorrhoeae and N. meningitidis in the human oropharynx. Surveillance programs are thus needed to monitor resistance in oropharyngeal commensal Neisseria, but currently the isolation and antimicrobial susceptibility testing of these commensals is laborious, complex and expensive. In addition, the posterior oropharyngeal/tonsillar swab, which is commonly used to sample oropharyngeal Neisseria, is poorly tolerated by many individuals. We evaluated an alternative non-invasive method to isolate oropharyngeal commensal Neisseria and to detect decreased susceptibility to azithromycin using selective media (LBVT.SNR) with and without azithromycin (2 µg/mL). In this pilot study, we compared paired posterior oropharyngeal/tonsillar swabs and oral rinse-and-gargle samples from 10 participants and demonstrated that a similar Neisseria species diversity and number of colonies were isolated from both sample types. Moreover, the proportion of Neisseria colonies that had a decreased susceptibility to azithromycin was similar in the rinse samples compared to the swabs. This pilot study has produced encouraging data that a simple protocol of oral rinse-and-gargle and culture on plates selective for commensal Neisseria with and without a target antimicrobial can be used as a surveillance tool to monitor antimicrobial susceptibility in commensal oropharyngeal Neisseria. Larger studies are required to validate these findings.
Keywords: Neisseria species; antimicrobial susceptibility; surveillance Neisseria species; antimicrobial susceptibility; surveillance

Share and Cite

MDPI and ACS Style

Laumen, J.G.E.; Abdellati, S.; Van Dijck, C.; Martiny, D.; De Baetselier, I.; Manoharan-Basil, S.S.; Van den Bossche, D.; Kenyon, C. A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria—A Pilot Study. Antibiotics 2022, 11, 100. https://doi.org/10.3390/antibiotics11010100

AMA Style

Laumen JGE, Abdellati S, Van Dijck C, Martiny D, De Baetselier I, Manoharan-Basil SS, Van den Bossche D, Kenyon C. A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria—A Pilot Study. Antibiotics. 2022; 11(1):100. https://doi.org/10.3390/antibiotics11010100

Chicago/Turabian Style

Laumen, Jolein Gyonne Elise, Saïd Abdellati, Christophe Van Dijck, Delphine Martiny, Irith De Baetselier, Sheeba Santhini Manoharan-Basil, Dorien Van den Bossche, and Chris Kenyon. 2022. "A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria—A Pilot Study" Antibiotics 11, no. 1: 100. https://doi.org/10.3390/antibiotics11010100

APA Style

Laumen, J. G. E., Abdellati, S., Van Dijck, C., Martiny, D., De Baetselier, I., Manoharan-Basil, S. S., Van den Bossche, D., & Kenyon, C. (2022). A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria—A Pilot Study. Antibiotics, 11(1), 100. https://doi.org/10.3390/antibiotics11010100

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop