Identification of Three Novel PmGRI1 Genomic Resistance Islands and One Multidrug Resistant Hybrid Structure of Tn7-like Transposon and PmGRI1 in Proteus mirabilis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains
2.2. Antimicrobial Susceptibility Testing
2.3. DNA Extraction
2.4. Whole Genome Sequencing and Analysis
3. Results and Discussion
3.1. Antibiotic Susceptibility and Detection of Antimicrobial Resistance Genes
3.2. Analysis of Three Novel Variants of PmGRI1 in P. mirabilis
3.3. Characteristics of the Hybrid Structure in P. mirabilis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strain | Source of Samples | SGI1/SGI1-like Gene Island Type | Antibiotic Resistance Genes a | Strain Resistance Profile | Size (bp) |
---|---|---|---|---|---|
HN31 | Swine | PmGRI1-HN31 | catA1, dfrA12, aadA2, sul1, mph(A), aacC2d, sul2, strA, strB, aphA1, floR, tet(A) | AMP-CHL-NAL-STR-SPT-GEN-TMP-SUL-SXT | 62,862 |
YN8 | Chicken | PmGRI1-YN8 | catA1, blaCTX-M-65, fosA3, sul1, aadA5, dfrA17, aadA1, dfrA1, sul2 | NAL-SPT-TMP-SUL-SXT | 62,784 |
YN9 | Chicken | PmGRI1-YN9 | catA1, blaCTX-M-65, fosA3, sul1, arr-3, catB3, blaOXA-1, aac(6′)-Ib-cr, blaTEM-1, floR, sul2, hph, aphA1a, strA, strB, aacC2d, aadA5, dfrA17, aadA1, dfrA1, aacC4 | AMP-CHL-FFC-NAL-CIP-STR-SPT-GEN-TMP-SUL-SXT | 55,239 |
HN2p | Swine | Hybrid structure of Tn7-like and PmGRI1 | rmtB, hph, aacC4, aphA1a, strA, strB, aadA1, aadA2, blaTEM-1b, qnrA1, lun(F), erm(42), catA1, floR, sul1, sul2, tet(G), dfrA12, dfrA1, blaCMY-2 | AMP-AMC-AMK-SXT-LEV-CIP-NAL-FFC-CHL-GEN | 123,622 |
HN31 | Swine | PmGRI1-HN31 | catA1, dfrA12, aadA2, sul1, mph(A), aacC2d, sul2, strA, strB, aphA1, floR, tet(A) | AMP-CHL-NAL-STR-SPT-GEN-TMP-SUL-SXT | 62,862 |
YN8 | Chicken | PmGRI1-YN8 | catA1, blaCTX-M-65, fosA3, sul1, aadA5, dfrA17, aadA1, dfrA1, sul2 | NAL-SPT-TMP-SUL-SXT | 62,784 |
YN9 | Chicken | PmGRI1-YN9 | catA1, blaCTX-M-65, fosA3, sul1, arr-3, catB3, blaOXA-1, aac(6′)-Ib-cr, blaTEM-1, floR, sul2, hph, aphA1a, strA, strB, aacC2d, aadA5, dfrA17, aadA1, dfrA1, aacC4 | AMP-CHL-FFC-NAL-CIP-STR-SPT-GEN-TMP-SUL-SXT | 55,239 |
HN2p | Swine | Hybrid structure of Tn7-like and PmGRI1 | rmtB, hph, aacC4, aphA1a, strA, strB, aadA1, aadA2, blaTEM-1b, qnrA1, lun(F), erm(42), catA1, floR, sul1, sul2, tet(G), dfrA12, dfrA1, blaCMY-2 | AMP-AMC-AMK-SXT-LEV-CIP-NAL-FFC-CHL-GEN | 123,622 |
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Ma, B.; Wang, X.; Lei, C.; Tang, Y.; He, J.; Gao, Y.; Zhang, Y.; Wang, H. Identification of Three Novel PmGRI1 Genomic Resistance Islands and One Multidrug Resistant Hybrid Structure of Tn7-like Transposon and PmGRI1 in Proteus mirabilis. Antibiotics 2021, 10, 1268. https://doi.org/10.3390/antibiotics10101268
Ma B, Wang X, Lei C, Tang Y, He J, Gao Y, Zhang Y, Wang H. Identification of Three Novel PmGRI1 Genomic Resistance Islands and One Multidrug Resistant Hybrid Structure of Tn7-like Transposon and PmGRI1 in Proteus mirabilis. Antibiotics. 2021; 10(10):1268. https://doi.org/10.3390/antibiotics10101268
Chicago/Turabian StyleMa, Boheng, Xuechun Wang, Changwei Lei, Yizhi Tang, Juan He, Yufeng Gao, Yu Zhang, and Hongning Wang. 2021. "Identification of Three Novel PmGRI1 Genomic Resistance Islands and One Multidrug Resistant Hybrid Structure of Tn7-like Transposon and PmGRI1 in Proteus mirabilis" Antibiotics 10, no. 10: 1268. https://doi.org/10.3390/antibiotics10101268