Next Article in Journal
Use of Electronic Noses for Diagnosis of Digestive and Respiratory Diseases through the Breath
Next Article in Special Issue
Waveguiding and SERS Simplified Raman Spectroscopy on Biological Samples
Previous Article in Journal
In-Vitro Characterization of mCerulean3_mRuby3 as a Novel FRET Pair with Favorable Bleed-Through Characteristics
Previous Article in Special Issue
The Recent Advances in Raman Microscopy and Imaging Techniques for Biosensors
Article Menu
Issue 1 (March) cover image

Export Article

Open AccessArticle
Biosensors 2019, 9(1), 34; https://doi.org/10.3390/bios9010034

Surface Enhanced Raman Spectroscopy of Lactoferrin Adsorbed on Silvered Porous Silicon Covered with Graphene

1
Laboratory of Applied Plasmonics, Belarusian State University of Informatics and Radioelectronics, 220013 Minsk, Belarus
2
Laboratory of Materials and Structures of Nanoelectronics, Belarusian State University of Informatics and Radioelectronics, 220013 Minsk, Belarus
3
Laboratory of Integrated Micro- and Nanosystems, Belarusian State University of Informatics and Radioelectronics, 220013 Minsk, Belarus
4
Laboratory of Nutrition and Sports Physiology, Institute of Physiology of the National Academy of Sciences of Belarus, 220072 Minsk, Belarus
5
Department of Biochemistry, Belarusian State University, 220030 Minsk, Belarus
6
Laboratory of Neutron Physics, Joint Institute for Nuclear Research, 141980 Dubna, Russia
7
Dubna State University, 141982 Dubna, Russia
*
Author to whom correspondence should be addressed.
Received: 17 December 2018 / Revised: 17 February 2019 / Accepted: 20 February 2019 / Published: 28 February 2019
(This article belongs to the Special Issue Applications of Raman Techniques in Biosensing)
  |  
PDF [4717 KB, uploaded 28 February 2019]
  |  

Abstract

We registered surface enhanced Raman scattering (SERS) spectra of the human lactoferrin molecules adsorbed on a silvered porous silicon (por-Si) from 10−6–10−18 M solutions. It was found that the por-Si template causes a negative surface potential of silver particles and their chemical resistivity to oxidation. These properties provided to attract positively charged lactoferrin molecules and prevent their interaction with metallic particles upon 473 nm laser excitation. The SERS spectra of lactoferrin adsorbed from 10−6 M solution were rather weak but a decrease of the concentration to 10−10 M led to an enormous growth of the SERS signal. This effect took place as oligomers of lactoferrin were broken down to monomeric units while its concentration was reduced. Oligomers are too large for a uniform overlap with electromagnetic field from silver particles. They cannot provide an intensive SERS signal from the top part of the molecules in contrast to monomers that can be completely covered by the electromagnetic field. The SERS spectra of lactoferrin at the 10−14 and 10−16 M concentrations were less intensive and started to change due to increasing contribution from the laser burned molecules. To prevent overheating the analyte molecules on the silvered por-Si were protected with graphene, which allowed the detection of lactoferrin adsorbed from the 10−18 M solution. View Full-Text
Keywords: surface enhanced Raman scattering; silver; porous silicon; graphene; lactoferrin; attomolar concentration surface enhanced Raman scattering; silver; porous silicon; graphene; lactoferrin; attomolar concentration
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Zavatski, S.; Khinevich, N.; Girel, K.; Redko, S.; Kovalchuk, N.; Komissarov, I.; Lukashevich, V.; Semak, I.; Mamatkulov, K.; Vorobyeva, M.; Arzumanyan, G.; Bandarenka, H. Surface Enhanced Raman Spectroscopy of Lactoferrin Adsorbed on Silvered Porous Silicon Covered with Graphene. Biosensors 2019, 9, 34.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top