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Biosensors 2019, 9(1), 24; https://doi.org/10.3390/bios9010024

Rapid Drop-Test for Lectin Binding with Glycopolymer-Coated Optical Ring Resonators

1
Fraunhofer Institute for Applied Polymer Research IAP, Geiselbergstr. 69, 14476 Potsdam, Germany
2
Fraunhofer Institute for Telecommunications, Heinrich Hertz Institute, HHI, Einsteinufer 37, 10587 Berlin, Germany
*
Author to whom correspondence should be addressed.
Received: 21 December 2018 / Revised: 25 January 2019 / Accepted: 6 February 2019 / Published: 12 February 2019
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Abstract

We fabricated a simple sensor system for qualitative analysis of glycan-mediated interactions. Our main aim was to establish a ronbbust system that allowes drop-tests without complex fluidics. The test system should be usable in routine analytics in the future and bear sufficient sensitivity to detect binding events in the nanomolar range. For this, we employed optical ring resonators and coated them with high avidity glycopolymers based on N-acetylglucosamine (GlcNAc). These hydrophilic polymers are also very feasible in preventing unspecific protein adsorption. Drop-on binding studies with suitable lectins showed that glycopolymers were specifically recognized by a lectin with GlcNAc-specificity and prevented unspecific protein interactions very well. The system could be elaborated in the future for detection of glycan-mediated interactions in the biomedical field and is promising in means of multiplexed analysis and usage in routine analysis. View Full-Text
Keywords: optical ring resonator; glycopolymers; lectins; glycan interactions optical ring resonator; glycopolymers; lectins; glycan interactions
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Schulte-Osseili, C.; Kleinert, M.; Keil, N.; Rosencrantz, R.R. Rapid Drop-Test for Lectin Binding with Glycopolymer-Coated Optical Ring Resonators. Biosensors 2019, 9, 24.

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