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Biosensors 2019, 9(1), 3; https://doi.org/10.3390/bios9010003

Simultaneous Detection of Dopamine and Serotonin—A Comparative Experimental and Theoretical Study of Neurotransmitter Interactions

1
Department of Physics, University of Texas at El Paso, El Paso, TX 79968, USA
2
Department of Biomedical Engineering, University of Texas at El Paso, El Paso, TX 79968, USA
3
Border Biomedical Research Center, University of Texas at El Paso, El Paso, TX 79968, USA
4
Department of Chemistry, University of Texas at El Paso, El Paso, TX 79968, USA
5
Division of Engineering, Department of Neurologic Surgery, Mayo Clinic, Rochester, MN 55905, USA
6
Department of Neurologic Surgery, Mayo Clinic, Rochester, MN 55905, USA
*
Author to whom correspondence should be addressed.
Received: 30 November 2018 / Revised: 19 December 2018 / Accepted: 21 December 2018 / Published: 26 December 2018
(This article belongs to the Special Issue Applications of Raman Techniques in Biosensing)
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Abstract

With the goal of accurately detecting and quantifying the amounts of dopamine (DA) and serotonin (5-HT) in mixtures of these neurotransmitters without using any labelling, we present a detailed, comparative computational and Raman experimental study. Although discrimination between these two analytes is achievable in such mixtures for concentrations in the millimolar range, their accurate quantification remains unattainable. As shown for the first time in this work, the formation of a new composite resulting from their interactions with each other is the main reason for this lack of quantification. While this new hydrogen-bonded complex further complicates potential analyte discrimination and quantification at concentrations characteristic of physiological levels (i.e., nanomolar concentrations), it can also open new avenues for its use in drug delivery and pharmaceutical research. This remark is based not only on chemical interactions analyzed here from both theoretical and experimental approaches, but also on biological relationship, with consideration of both functional and neural proximity perspectives. Thus, this research constitutes an important contribution toward better understanding of neural processes, as well as toward possible future development of label-free biosensors. View Full-Text
Keywords: surface-enhanced Raman spectroscopy; neurotransmitters; dopamine; serotonin; computational analysis; simultaneous detection; label-free optical biosensors surface-enhanced Raman spectroscopy; neurotransmitters; dopamine; serotonin; computational analysis; simultaneous detection; label-free optical biosensors
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Manciu, F.S.; Manciu, M.; Ciubuc, J.D.; Sundin, E.M.; Ochoa, K.; Eastman, M.; Durrer, W.G.; Guerrero, J.; Lopez, B.; Subedi, M.; Bennet, K.E. Simultaneous Detection of Dopamine and Serotonin—A Comparative Experimental and Theoretical Study of Neurotransmitter Interactions. Biosensors 2019, 9, 3.

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