Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis
AbstractA new method using a magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was compared with sputum smear microscopy (SSM) for the detection of pulmonary tuberculosis (PTB) in sputum samples. Studies were made to compare the NCBA against SSM using sputum samples collected from PTB patients prior to receiving treatment. Experiments were also conducted to determine the appropriate concentration of glycan-functionalized magnetic nanoparticles (GMNP) used in the NCBA and to evaluate the optimal digestion/decontamination solution to increase the extraction, concentration and detection of acid-fast bacilli (AFB). The optimized NCBA consisted of a 1:1 mixture of 0.4% NaOH and 4% N-acetyl-L-cysteine (NALC) to homogenize the sputum sample. Additionally, 10 mg/mL of GMNP was added to isolate and concentrate the AFB. All TB positive sputum samples were identified with an increased AFB count of 47% compared to SSM, demonstrating GMNP’s ability to extract and concentrate AFB. Results showed that NCBA increased AFB count compared to SSM, improving the grade from “1+” (in SSM) to “2+”. Extending the finding to paucibacillary cases, there is the likelihood of a “scant” grade to become “1+”. The assay uses a simple magnet and only costs $0.10/test. NCBA has great potential application in TB control programs. View Full-Text
Share & Cite This Article
Gordillo-Marroquín, C.; Gómez-Velasco, A.; Sánchez-Pérez, H.J.; Pryg, K.; Shinners, J.; Murray, N.; Muñoz-Jiménez, S.G.; Bencomo-Alerm, A.; Gómez-Bustamante, A.; Jonapá-Gómez, L.; Enríquez-Ríos, N.; Martín, M.; Romero-Sandoval, N.; Alocilja, E.C. Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis. Biosensors 2018, 8, 128.
Gordillo-Marroquín C, Gómez-Velasco A, Sánchez-Pérez HJ, Pryg K, Shinners J, Murray N, Muñoz-Jiménez SG, Bencomo-Alerm A, Gómez-Bustamante A, Jonapá-Gómez L, Enríquez-Ríos N, Martín M, Romero-Sandoval N, Alocilja EC. Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis. Biosensors. 2018; 8(4):128.Chicago/Turabian Style
Gordillo-Marroquín, Cristina; Gómez-Velasco, Anaximandro; Sánchez-Pérez, Héctor J.; Pryg, Kasey; Shinners, John; Murray, Nathan; Muñoz-Jiménez, Sergio G.; Bencomo-Alerm, Allied; Gómez-Bustamante, Adriana; Jonapá-Gómez, Letisia; Enríquez-Ríos, Natán; Martín, Miguel; Romero-Sandoval, Natalia; Alocilja, Evangelyn C. 2018. "Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis." Biosensors 8, no. 4: 128.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.