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Volume 15
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10.3390/bios15040264
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Article

Point-of-Care NSE Biosensor for Objective Assessment of Stroke Risk

by
Cheng Hsu Chen
1,2,†,
Erick Wang
3,4,†,
Tsung-Han Lee
1,5,6,
Cheng-Chieh Huang
1,2,
Chun-San Tai
4,
Yan-Ren Lin
1,5,7,* and
Wen-Liang Chen
2,3,8,9,*
1
Department of Emergency and Critical Care Medicine, Changhua Christian Hospital, Changhua 50006, Taiwan
2
Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan
3
Industrial Development Graduate Program of College of Engineering and Bioscience, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan
4
AgriTalk Technology Inc., Hsinchu 30010, Taiwan
5
Department of Post Baccalaureate Medicine, College of Medicine, National Chung Hsing University, Taichung 40227, Taiwan
6
Department of Nursing, Chang Jung Christian University, Tainan 71101, Taiwan
7
School of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan
8
Graduate Degree Program of Biomedical Science and Engineering, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan
9
Center for Regenerative Medicine and Cellular Therapy, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Biosensors 2025, 15(4), 264; https://doi.org/10.3390/bios15040264
Submission received: 25 February 2025 / Revised: 6 April 2025 / Accepted: 17 April 2025 / Published: 20 April 2025
(This article belongs to the Special Issue Applications of Cutting-Edge Biosensors in Environment, Food and Healthcare Field)
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Abstract

The rapid identification of stroke is critical to improving stroke patient outcomes. Existing protocols for assessing the risk of stroke are subjective and may be further complicated by nonspecific symptoms, increasing the risk of misdiagnosis. Neuron-specific enolase (NSE) has emerged as a promising stroke biomarker. However, current detection methods such as the electrochemiluminescence immunoassay (ECLIA) are time-consuming and costly. In this research, we developed an electrochemical biosensor for the rapid quantification of NSE in whole blood. Mouse stroke models were established, and blood samples collected were analyzed using both hospital-standard ECLIA as well as the biosensor. The biosensor limit of detection was 1.15 ng/mL. NSE measurements were highly correlated between the two methods and were obtained in 5 min using 20 μL of unprocessed whole blood samples. Notably, the biosensor could accurately quantify elevated blood NSE blood that was associated with more severe stroke. Our results demonstrate the utility of the proposed biosensor in pre-hospital settings. Combined with existing stroke assessment methods, the biosensor may enable emergency personnel to identify stroke risk with greater accuracy to optimize the chances of receiving necessary treatment within the effective window.
Keywords: neuron-specific enolase; stroke assessment; stroke biomarker neuron-specific enolase; stroke assessment; stroke biomarker

Share and Cite

MDPI and ACS Style

Hsu Chen, C.; Wang, E.; Lee, T.-H.; Huang, C.-C.; Tai, C.-S.; Lin, Y.-R.; Chen, W.-L. Point-of-Care NSE Biosensor for Objective Assessment of Stroke Risk. Biosensors 2025, 15, 264. https://doi.org/10.3390/bios15040264

AMA Style

Hsu Chen C, Wang E, Lee T-H, Huang C-C, Tai C-S, Lin Y-R, Chen W-L. Point-of-Care NSE Biosensor for Objective Assessment of Stroke Risk. Biosensors. 2025; 15(4):264. https://doi.org/10.3390/bios15040264

Chicago/Turabian Style

Hsu Chen, Cheng, Erick Wang, Tsung-Han Lee, Cheng-Chieh Huang, Chun-San Tai, Yan-Ren Lin, and Wen-Liang Chen. 2025. "Point-of-Care NSE Biosensor for Objective Assessment of Stroke Risk" Biosensors 15, no. 4: 264. https://doi.org/10.3390/bios15040264

APA Style

Hsu Chen, C., Wang, E., Lee, T.-H., Huang, C.-C., Tai, C.-S., Lin, Y.-R., & Chen, W.-L. (2025). Point-of-Care NSE Biosensor for Objective Assessment of Stroke Risk. Biosensors, 15(4), 264. https://doi.org/10.3390/bios15040264

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