Quantitation of MicroRNA-155 in Human Cells by Heterogeneous Enzyme-Linked Oligonucleotide Assay Coupled with Mismatched Catalytic Hairpin Assembly Reaction
Abstract
:1. Introduction
2. Experimental
2.1. Reagents, Materials, and Equipment
2.2. Measurement of MicroRNA Concentration
2.3. Purification and Quantitation of Cellular MicroRNAs
3. Results and Discussion
3.1. Design of the MicroRNA Assay
3.2. Optimization of the Assay Conditions for MicroRNA-155 Detection
3.3. Analytical Parameters of the Heterogenous mCHA-Based Assay of MicroRNA-155
3.4. Specificity
3.5. Detection of MicroRNAs in Human Cells
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Bodulev, O.L.; Galkin, I.I.; Zhao, S.; Pletyushkina, O.Y.; Sakharov, I.Y. Quantitation of MicroRNA-155 in Human Cells by Heterogeneous Enzyme-Linked Oligonucleotide Assay Coupled with Mismatched Catalytic Hairpin Assembly Reaction. Biosensors 2022, 12, 570. https://doi.org/10.3390/bios12080570
Bodulev OL, Galkin II, Zhao S, Pletyushkina OY, Sakharov IY. Quantitation of MicroRNA-155 in Human Cells by Heterogeneous Enzyme-Linked Oligonucleotide Assay Coupled with Mismatched Catalytic Hairpin Assembly Reaction. Biosensors. 2022; 12(8):570. https://doi.org/10.3390/bios12080570
Chicago/Turabian StyleBodulev, Oleg L., Ivan I. Galkin, Shulin Zhao, Olga Y. Pletyushkina, and Ivan Y. Sakharov. 2022. "Quantitation of MicroRNA-155 in Human Cells by Heterogeneous Enzyme-Linked Oligonucleotide Assay Coupled with Mismatched Catalytic Hairpin Assembly Reaction" Biosensors 12, no. 8: 570. https://doi.org/10.3390/bios12080570