Solid-Phase Synthesis of Red Fluorescent Carbon Dots for the Dual-Mode Detection of Hexavalent Chromium and Cell Imaging

Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China

Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, China

Institute of Advanced Materials, Jiangxi Normal University, Nanchang 330022, China

Authors to whom correspondence should be addressed.

Biosensors 2022, 12(6), 432; https://doi.org/10.3390/bios12060432

Received: 15 May 2022 / Revised: 1 June 2022 / Accepted: 18 June 2022 / Published: 20 June 2022

(This article belongs to the Special Issue Application of Nanomaterials for Biosensors)

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Abstract

The excellent optical properties and biocompatibility of red fluorescence carbon dots (R-CDs) provide a new approach for the effective analysis of hexavalent chromium Cr(VI) in environmental and biological samples. However, the application of R-CDs is still limited by low yield and unfriendly synthesis route. In this study, we developed a new type of R-CDs based on a simple and green solid-phase preparation strategy. The synthesized R-CDs can emit bright red fluorescence with an emission wavelength of 625 nm and also have an obvious visible light absorption capacity. Furthermore, the absorption and fluorescence signals of the R-CDs aqueous solution are sensitive to Cr(VI), which is reflected in color change and fluorescence quenching. Based on that, a scanometric and fluorescent dual-mode analysis system for the rapid and accurate detection of Cr(VI) was established well within the limit of detection at 80 nM and 9.1 nM, respectively. The proposed methods also possess high specificity and were applied for the detection of Cr(VI) in real water samples. More importantly, the synthesized R-CDs with good biocompatibility were further successfully applied for visualizing intracellular Cr(VI) in Hela cells. View Full-Text

Keywords: solid-phase synthesis; R-CDs; scanometric and fluorescent; Cr(VI) detection

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MDPI and ACS Style

Hu, J.; Wang, X.; Wei, H.; Zhao, L.; Yao, B.; Zhang, C.; Zhou, J.; Liu, J.; Yang, S. Solid-Phase Synthesis of Red Fluorescent Carbon Dots for the Dual-Mode Detection of Hexavalent Chromium and Cell Imaging. Biosensors 2022, 12, 432. https://doi.org/10.3390/bios12060432

AMA Style

Hu J, Wang X, Wei H, Zhao L, Yao B, Zhang C, Zhou J, Liu J, Yang S. Solid-Phase Synthesis of Red Fluorescent Carbon Dots for the Dual-Mode Detection of Hexavalent Chromium and Cell Imaging. Biosensors. 2022; 12(6):432. https://doi.org/10.3390/bios12060432

Chicago/Turabian Style

Hu, Jinshuang, Xin Wang, Hua Wei, Lei Zhao, Boxuan Yao, Caiyun Zhang, Jiarui Zhou, Jian Liu, and Shenghong Yang. 2022. "Solid-Phase Synthesis of Red Fluorescent Carbon Dots for the Dual-Mode Detection of Hexavalent Chromium and Cell Imaging" Biosensors 12, no. 6: 432. https://doi.org/10.3390/bios12060432

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Solid-Phase Synthesis of Red Fluorescent Carbon Dots for the Dual-Mode Detection of Hexavalent Chromium and Cell Imaging

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