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Open AccessArticle

Development of a Ratiometric Fluorescent Glucose Sensor Using an Oxygen-Sensing Membrane Immobilized with Glucose Oxidase for the Detection of Glucose in Tears

1
School of Chemical Engineering and Research Center for Biophotonics, Chonnam National University, Gwangju 61186, Korea
2
JoinTree Co., GwangJu 62421, Korea
*
Author to whom correspondence should be addressed.
Biosensors 2020, 10(8), 86; https://doi.org/10.3390/bios10080086
Received: 7 July 2020 / Revised: 28 July 2020 / Accepted: 28 July 2020 / Published: 29 July 2020
(This article belongs to the Special Issue Fluorescence Biosensors 2020)
Glucose concentration is an important parameter in biomedicine since glucose is involved in many metabolic pathways in organisms. Many methods for glucose detection have been developed for use in various applications, particularly in the field of healthcare in diabetics. In this study, ratiometric fluorescent glucose-sensing membranes were fabricated based on the oxygen levels consumed in the glucose oxidation reaction under the catalysis of glucose oxidase (GOD). The oxygen concentration was measured through the fluorescence quenching effect of an oxygen-sensitive fluorescent dye like platinum meso-tetra (pentafluorophenyl) porphyrin (PtP) by oxygen molecules. Coumarin 6 (C6) was used as a reference dye in the ratiometric fluorescence measurements. The glucose-sensing membrane consisted of two layers: The first layer was the oxygen-sensing membrane containing polystyrene particles (PS) doped with PtP and C6 (e.g., [email protected]^PtP) in a sol–gel matrix of aminopropyltrimethoxysilane and glycidoxypropyltrimethoxysilane (GA). The second layer was made by immobilizing GOD onto one of three supporting polymers over the first layer. These glucose-sensing membranes were characterized in terms of their response, reversibility, interferences, and stability. They showed a wide detection range to glucose concentration in the range of 0.1 to 10 mM, but high sensitivity with a linear detection range of 0.1 to 2 mM glucose. This stable and sensitive ratiometric fluorescent glucose biosensor provides a reliable way to determine low glucose concentrations in blood serum by measuring tear glucose. View Full-Text
Keywords: glucose-sensing membrane; glucose oxidase; ratiometric fluorescent sensor; fluorescence quenching; tear glucose glucose-sensing membrane; glucose oxidase; ratiometric fluorescent sensor; fluorescence quenching; tear glucose
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MDPI and ACS Style

Duong, H.D.; Sohn, O.-J.; Rhee, J.I. Development of a Ratiometric Fluorescent Glucose Sensor Using an Oxygen-Sensing Membrane Immobilized with Glucose Oxidase for the Detection of Glucose in Tears. Biosensors 2020, 10, 86. https://doi.org/10.3390/bios10080086

AMA Style

Duong HD, Sohn O-J, Rhee JI. Development of a Ratiometric Fluorescent Glucose Sensor Using an Oxygen-Sensing Membrane Immobilized with Glucose Oxidase for the Detection of Glucose in Tears. Biosensors. 2020; 10(8):86. https://doi.org/10.3390/bios10080086

Chicago/Turabian Style

Duong, Hong D.; Sohn, Ok-Jae; Rhee, Jong I. 2020. "Development of a Ratiometric Fluorescent Glucose Sensor Using an Oxygen-Sensing Membrane Immobilized with Glucose Oxidase for the Detection of Glucose in Tears" Biosensors 10, no. 8: 86. https://doi.org/10.3390/bios10080086

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