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Article

Development of a Rapid, Antimicrobial Susceptibility Test for E. coli Based on Low-Cost, Screen-Printed Electrodes

1
Department of Biomedical Engineering, University of Strathclyde, 40 George Street, Glasgow G1 1QE, UK
2
Division of Anaesthesia, Royal Alexandra Hospital, Corsebar Road, Paisley PA2 9PN, UK
3
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, UK
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Biosensors 2020, 10(11), 153; https://doi.org/10.3390/bios10110153
Received: 29 September 2020 / Revised: 20 October 2020 / Accepted: 22 October 2020 / Published: 23 October 2020
(This article belongs to the Section Biosensors and Healthcare)
Antibiotic resistance has been cited by the World Health Organisation (WHO) as one of the greatest threats to public health. Mitigating the spread of antibiotic resistance requires a multipronged approach with possible interventions including faster diagnostic testing and enhanced antibiotic stewardship. This study employs a low-cost diagnostic sensor test to rapidly pinpoint the correct antibiotic for treatment of infection. The sensor comprises a screen-printed gold electrode, modified with an antibiotic-seeded hydrogel to monitor bacterial growth. Electrochemical growth profiles of the common microorganism, Escherichia coli (E. coli) (ATCC 25922) were measured in the presence and absence of the antibiotic streptomycin. Results show a clear distinction between the E. coli growth profiles depending on whether streptomycin is present, in a timeframe of ≈2.5 h (p < 0.05), significantly quicker than the current gold standard of culture-based antimicrobial susceptibility testing. These results demonstrate a clear pathway to a low cost, phenotypic and reproducible antibiotic susceptibility testing technology for the rapid detection of E. coli within clinically relevant concentration ranges for conditions such as urinary tract infections. View Full-Text
Keywords: antibiotic susceptibility testing (AST); Escherichia coli (E. coli); electrochemistry; screen-printed electrodes (SPEs); streptomycin; growth-profiles; real-time monitoring antibiotic susceptibility testing (AST); Escherichia coli (E. coli); electrochemistry; screen-printed electrodes (SPEs); streptomycin; growth-profiles; real-time monitoring
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MDPI and ACS Style

Hannah, S.; Dobrea, A.; Lasserre, P.; Blair, E.O.; Alcorn, D.; Hoskisson, P.A.; Corrigan, D.K. Development of a Rapid, Antimicrobial Susceptibility Test for E. coli Based on Low-Cost, Screen-Printed Electrodes. Biosensors 2020, 10, 153. https://doi.org/10.3390/bios10110153

AMA Style

Hannah S, Dobrea A, Lasserre P, Blair EO, Alcorn D, Hoskisson PA, Corrigan DK. Development of a Rapid, Antimicrobial Susceptibility Test for E. coli Based on Low-Cost, Screen-Printed Electrodes. Biosensors. 2020; 10(11):153. https://doi.org/10.3390/bios10110153

Chicago/Turabian Style

Hannah, Stuart, Alexandra Dobrea, Perrine Lasserre, Ewen O. Blair, David Alcorn, Paul A. Hoskisson, and Damion K. Corrigan. 2020. "Development of a Rapid, Antimicrobial Susceptibility Test for E. coli Based on Low-Cost, Screen-Printed Electrodes" Biosensors 10, no. 11: 153. https://doi.org/10.3390/bios10110153

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