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Scaffold Structural Microenvironmental Cues to Guide Tissue Regeneration in Bone Tissue Applications
Article

Injectable Alginate-Peptide Composite Hydrogel as a Scaffold for Bone Tissue Regeneration

Department of Oral Biology, The Goldschleger School of Dental Medicine, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 6997801, Israel
*
Author to whom correspondence should be addressed.
Those authors contributed equally to this work.
Nanomaterials 2019, 9(4), 497; https://doi.org/10.3390/nano9040497
Received: 11 February 2019 / Revised: 17 March 2019 / Accepted: 23 March 2019 / Published: 1 April 2019
(This article belongs to the Special Issue Nanoscale Materials and Technologies in Tissue Engineering)
The high demand for tissue engineering scaffolds capable of inducing bone regeneration using minimally invasive techniques prompts the need for the development of new biomaterials. Herein, we investigate the ability of Alginate incorporated with the fluorenylmethoxycarbonyl-diphenylalanine (FmocFF) peptide composite hydrogel to serve as a potential biomaterial for bone regeneration. We demonstrate that the incorporation of the self-assembling peptide, FmocFF, in sodium alginate leads to the production of a rigid, yet injectable, hydrogel without the addition of cross-linking agents. Scanning electron microscopy reveals a nanofibrous structure which mimics the natural bone extracellular matrix. The formed composite hydrogel exhibits thixotropic behavior and a high storage modulus of approximately 10 kPA, as observed in rheological measurements. The in vitro biocompatibility tests carried out with MC3T3-E1 preosteoblast cells demonstrate good cell viability and adhesion to the hydrogel fibers. This composite scaffold can induce osteogenic differentiation and facilitate calcium mineralization, as shown by Alizarin red staining, alkaline phosphatase activity and RT-PCR analysis. The high biocompatibility, excellent mechanical properties and similarity to the native extracellular matrix suggest the utilization of this hydrogel as a temporary three-dimensional cellular microenvironment promoting bone regeneration. View Full-Text
Keywords: regenerative medicine; scaffolds; nanomaterials; extracellular matrix; hydrogels; self-assembly regenerative medicine; scaffolds; nanomaterials; extracellular matrix; hydrogels; self-assembly
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MDPI and ACS Style

Ghosh, M.; Halperin-Sternfeld, M.; Grinberg, I.; Adler-Abramovich, L. Injectable Alginate-Peptide Composite Hydrogel as a Scaffold for Bone Tissue Regeneration. Nanomaterials 2019, 9, 497. https://doi.org/10.3390/nano9040497

AMA Style

Ghosh M, Halperin-Sternfeld M, Grinberg I, Adler-Abramovich L. Injectable Alginate-Peptide Composite Hydrogel as a Scaffold for Bone Tissue Regeneration. Nanomaterials. 2019; 9(4):497. https://doi.org/10.3390/nano9040497

Chicago/Turabian Style

Ghosh, Moumita, Michal Halperin-Sternfeld, Itzhak Grinberg, and Lihi Adler-Abramovich. 2019. "Injectable Alginate-Peptide Composite Hydrogel as a Scaffold for Bone Tissue Regeneration" Nanomaterials 9, no. 4: 497. https://doi.org/10.3390/nano9040497

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