Next Article in Journal
“Chocolate” Gold Nanoparticles—One Pot Synthesis and Biocompatibility
Next Article in Special Issue
Lanthanide-Doped Upconversion Nanocarriers for Drug and Gene Delivery
Previous Article in Journal
Controlling the 3D Electromagnetic Coupling in Co-Sputtered Ag–SiO2 Nanomace Arrays by Lateral Sizes
Previous Article in Special Issue
Evaluation of the PEG Density in the PEGylated Chitosan Nanoparticles as a Drug Carrier for Curcumin and Mitoxantrone
Article Menu
Issue 7 (July) cover image

Export Article

Open AccessArticle
Nanomaterials 2018, 8(7), 495;

Coating Dependent In Vitro Biocompatibility of New Fe-Si Nanoparticles

Department of Biochemistry and Molecular Biology, University of Bucharest, 91–95 Splaiul Independenţei, 050095 Bucharest, sector 5, Romania
National Institute for Lasers, Plasma and Radiation Physics (NILPRP), Atomistilor 409, 077125 Magurele, Romania
Department of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Gh. Polizu 1-7, 11061 Bucharest, sector 1, Romania
Author to whom correspondence should be addressed.
Received: 24 May 2018 / Revised: 28 June 2018 / Accepted: 2 July 2018 / Published: 5 July 2018
(This article belongs to the Special Issue Biomedical Applications of Nanoparticles)
Full-Text   |   PDF [3638 KB, uploaded 5 July 2018]   |  


Magnetic nanoparticles offer multiple utilization possibilities in biomedicine. In this context, the interaction with cellular structures and their biological effects need to be understood and controlled for clinical safety. New magnetic nanoparticles containing metallic/carbidic iron and elemental silicon phases were synthesized by laser pyrolysis using Fe(CO)5 vapors and SiH4 gas as Fe and Si precursors, then passivated and coated with biocompatible agents, such as l-3,4-dihydroxyphenylalanine (l-DOPA) and sodium carboxymethyl cellulose (CMC-Na). The resulting magnetic nanoparticles were characterized by XRD, EDS, and TEM techniques. To evaluate their biocompatibility, doses ranging from 0–200 µg/mL hybrid Fe-Si nanoparticles were exposed to Caco2 cells for 24 and 72 h. Doses below 50 μg/mL of both l-DOPA and CMC-Na-coated Fe-Si nanoparticles induced no significant changes of cellular viability or membrane integrity. The cellular internalization of nanoparticles was dependent on their dispersion in culture medium and caused some changes of F-actin filaments organization after 72 h. However, reactive oxygen species were generated after exposure to 25 and 50 μg/mL of both Fe-Si nanoparticles types, inducing the increase of intracellular glutathione level and activation of transcription factor Nrf2. At nanoparticles doses below 50 μg/mL, Caco2 cells were able to counteract the oxidative stress by activating the cellular protection mechanisms. We concluded that in vitro biological responses to coated hybrid Fe-Si nanoparticles depended on particle synthesis conditions, surface coating, doses and incubation time. View Full-Text
Keywords: hybrid Fe-Si nanoparticles; laser pyrolysis; Caco2 cells; cytotoxicity; oxidative stress hybrid Fe-Si nanoparticles; laser pyrolysis; Caco2 cells; cytotoxicity; oxidative stress

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Balas, M.; Dumitrache, F.; Badea, M.A.; Fleaca, C.; Badoi, A.; Tanasa, E.; Dinischiotu, A. Coating Dependent In Vitro Biocompatibility of New Fe-Si Nanoparticles. Nanomaterials 2018, 8, 495.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Nanomaterials EISSN 2079-4991 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top