Next Article in Journal
Porous α-Fe2O3@C Nanowire Arrays as Flexible Supercapacitors Electrode Materials with Excellent Electrochemical Performances
Next Article in Special Issue
Coating Dependent In Vitro Biocompatibility of New Fe-Si Nanoparticles
Previous Article in Journal
Broadband Perfect Absorber Based on TiN-Nanocone Metasurface
Article

Evaluation of the PEG Density in the PEGylated Chitosan Nanoparticles as a Drug Carrier for Curcumin and Mitoxantrone

Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, School of Medicine, Hunan Normal University, Changsha 410013, China
*
Author to whom correspondence should be addressed.
Nanomaterials 2018, 8(7), 486; https://doi.org/10.3390/nano8070486
Received: 15 May 2018 / Revised: 5 June 2018 / Accepted: 8 June 2018 / Published: 1 July 2018
(This article belongs to the Special Issue Biomedical Applications of Nanoparticles)
Polyethylene glycolated (PEGylated)curcumin-grafted-chitosan (PCC) conjugates were synthesized with three PEG/chitosan feed molar ratios (1/5, 1/7.5, and 1/10), namely PCC1, PCC2 and PCC3. Chemical structures of these conjugates were characterized by Fourier transform infrared (FTIR) and proton nuclear magnetic resonance (1H NMR). The degrees of substitution (DS) of PEG were 0.75%, 0.45% and 0.33%, respectively, for PCC1, PCC2 and PCC3by 1H NMR analysis. Self-assembled PCC nanoparticles (NPs) were spherical as observed in transmission electron microscope images. Mitoxantrone (MTO)-loaded PCC NPs were prepared to analyze the particle size, zeta potential, drug loading, drug release and in vitro cytotoxicity. The MTO-loaded PCC3 NP (DS = 0.33%) possessed the smallest size (~183.1 nm), highest zeta potential (~+34.0 mV) and the largest loading capacity of curcumin (CUR, ~16.1%) and MTO (~8.30%). The release results showed that MTO-loaded PCC3 NP demonstrated the lowest percentage of MTO release and increased as pH decreased, but the CUR release could only be detected at pH 4.0. In the cytotoxicity study, MTO-loaded PCC3 NP displayed the highest cytotoxicity in HepG2 cell line and the best synergistic effect among the tested NPs. Our results suggest that the DS of PEG has impacts on the structures and functions of PCC NPs: the smaller DS of PEG was associated with the smaller size, the higher zeta potential, the slower drug release, and the higher cytotoxicity of NPs. View Full-Text
Keywords: curcumin; mitoxantrone; synergism; PEG; chitosan nanoparticles curcumin; mitoxantrone; synergism; PEG; chitosan nanoparticles
Show Figures

Figure 1

MDPI and ACS Style

Chen, Y.; Wu, D.; Zhong, W.; Kuang, S.; Luo, Q.; Song, L.; He, L.; Feng, X.; Tao, X. Evaluation of the PEG Density in the PEGylated Chitosan Nanoparticles as a Drug Carrier for Curcumin and Mitoxantrone. Nanomaterials 2018, 8, 486. https://doi.org/10.3390/nano8070486

AMA Style

Chen Y, Wu D, Zhong W, Kuang S, Luo Q, Song L, He L, Feng X, Tao X. Evaluation of the PEG Density in the PEGylated Chitosan Nanoparticles as a Drug Carrier for Curcumin and Mitoxantrone. Nanomaterials. 2018; 8(7):486. https://doi.org/10.3390/nano8070486

Chicago/Turabian Style

Chen, Yao, Di Wu, Wu Zhong, Shuwen Kuang, Qian Luo, Liujiang Song, Lihua He, Xing Feng, and Xiaojun Tao. 2018. "Evaluation of the PEG Density in the PEGylated Chitosan Nanoparticles as a Drug Carrier for Curcumin and Mitoxantrone" Nanomaterials 8, no. 7: 486. https://doi.org/10.3390/nano8070486

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop