Controlled Release of the α-Tocopherol-Derived Metabolite α-13′-Carboxychromanol from Bacterial Nanocellulose Wound Cover Improves Wound Healing
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation, Semi-Synthesis, and Dilution of Metabolites
2.2. Animals
2.3. Diabetes Mouse Model
2.4. Splinted Wound Model
2.5. Histopathological Analysis
2.6. Collagen Quantification by Second Harmonic Generation Imaging
2.7. Lipid Mediator Analysis Using UPLC-MS/MS
2.8. Cell Culture
2.9. Wound Healing Environment
2.10. RNA Isolation and cDNA Synthesis
2.11. Quantitative Real-Time RT-qPCR
2.12. Scratch Assay
2.13. Preparation of Bacterial Nanocellulose
2.14. Dimensions of the Bacterial Nanocellulose
2.15. Loading of Bacterial Nanocellulose
2.16. Moisture Retention Capacity
2.17. Statistics
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hoff, J.; Karl, B.; Gerstmeier, J.; Beekmann, U.; Schmölz, L.; Börner, F.; Kralisch, D.; Bauer, M.; Werz, O.; Fischer, D.; et al. Controlled Release of the α-Tocopherol-Derived Metabolite α-13′-Carboxychromanol from Bacterial Nanocellulose Wound Cover Improves Wound Healing. Nanomaterials 2021, 11, 1939. https://doi.org/10.3390/nano11081939
Hoff J, Karl B, Gerstmeier J, Beekmann U, Schmölz L, Börner F, Kralisch D, Bauer M, Werz O, Fischer D, et al. Controlled Release of the α-Tocopherol-Derived Metabolite α-13′-Carboxychromanol from Bacterial Nanocellulose Wound Cover Improves Wound Healing. Nanomaterials. 2021; 11(8):1939. https://doi.org/10.3390/nano11081939
Chicago/Turabian StyleHoff, Jessica, Berit Karl, Jana Gerstmeier, Uwe Beekmann, Lisa Schmölz, Friedemann Börner, Dana Kralisch, Michael Bauer, Oliver Werz, Dagmar Fischer, and et al. 2021. "Controlled Release of the α-Tocopherol-Derived Metabolite α-13′-Carboxychromanol from Bacterial Nanocellulose Wound Cover Improves Wound Healing" Nanomaterials 11, no. 8: 1939. https://doi.org/10.3390/nano11081939