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Article

In Vitro Co-Exposure to CeO2 Nanomaterials from Diesel Engine Exhaust and Benzo(a)Pyrene Induces Additive DNA Damage in Sperm and Cumulus Cells but Not in Oocytes

1
IMBE, CNRS, IRD, Avignon Université, Aix Marseille Univ., 13005 Marseille, France
2
CEREGE, CNRS, Aix Marseille Univ., IRD, INRAE, Coll France, 13545 Aix-en-Provence, France
3
Civil and Environmental Engineering, Duke University, Durham, NC 27708, USA
4
Department of Biostatistics and Public Health, La Timone Hospital, 13005 Marseille, France
5
Laboratory of Reproduction Biology-CECOS, Department of Gynecology, Obstetrics and Reproductive Medicine, AP-HM La Conception, Pôle Femmes Parents Enfants, 13005 Marseille, France
*
Author to whom correspondence should be addressed.
Academic Editor: Saura Sahu
Nanomaterials 2021, 11(2), 478; https://doi.org/10.3390/nano11020478
Received: 21 January 2021 / Revised: 3 February 2021 / Accepted: 7 February 2021 / Published: 13 February 2021
(This article belongs to the Special Issue Advances in Toxicity of Nanoparticles)
Benzo(a)pyrene (BaP) is a recognized reprotoxic compound and the most widely investigated polycyclic aromatic hydrocarbon in ambient air; it is widespread by the incomplete combustion of fossil fuels along with cerium dioxide nanomaterials (CeO2 NMs), which are used in nano-based diesel additives to decrease the emission of toxic compounds and to increase fuel economy. The toxicity of CeO2 NMs on reproductive organs and cells has also been shown. However, the effect of the combined interactions of BaP and CeO2 NMs on reproduction has not been investigated. Herein, human and rat gametes were exposed in vitro to combusted CeO2 NMs or BaP or CeO2 NMs and BaP in combination. CeO2 NMs were burned at 850 °C prior to mimicking their release after combustion in a diesel engine. We demonstrated significantly higher amounts of DNA damage after exposure to combusted CeO2 NMs (1 µg·L−1) or BaP (1.13 µmol·L−1) in all cell types considered compared to unexposed cells. Co-exposure to the CeO2 NMs-BaP mixture induced additive DNA damage in sperm and cumulus cells, whereas no additive effect was observed in rat oocytes. This result could be related to the structural protection of the oocyte by cumulus cells and to the oocyte’s efficient system to repair DNA damage compared to that of cumulus and sperm cells. View Full-Text
Keywords: genotoxicity; nanomaterials; polycyclic aromatic hydrocarbons; germ cells; additivity; cocktail genotoxicity; nanomaterials; polycyclic aromatic hydrocarbons; germ cells; additivity; cocktail
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MDPI and ACS Style

Cotena, M.; Auffan, M.; Tassistro, V.; Resseguier, N.; Rose, J.; Perrin, J. In Vitro Co-Exposure to CeO2 Nanomaterials from Diesel Engine Exhaust and Benzo(a)Pyrene Induces Additive DNA Damage in Sperm and Cumulus Cells but Not in Oocytes. Nanomaterials 2021, 11, 478. https://doi.org/10.3390/nano11020478

AMA Style

Cotena M, Auffan M, Tassistro V, Resseguier N, Rose J, Perrin J. In Vitro Co-Exposure to CeO2 Nanomaterials from Diesel Engine Exhaust and Benzo(a)Pyrene Induces Additive DNA Damage in Sperm and Cumulus Cells but Not in Oocytes. Nanomaterials. 2021; 11(2):478. https://doi.org/10.3390/nano11020478

Chicago/Turabian Style

Cotena, Martina, Mélanie Auffan, Virginie Tassistro, Noémie Resseguier, Jérôme Rose, and Jeanne Perrin. 2021. "In Vitro Co-Exposure to CeO2 Nanomaterials from Diesel Engine Exhaust and Benzo(a)Pyrene Induces Additive DNA Damage in Sperm and Cumulus Cells but Not in Oocytes" Nanomaterials 11, no. 2: 478. https://doi.org/10.3390/nano11020478

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