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Open AccessArticle

Ultrastructural Features of Gold Nanoparticles Interaction with HepG2 and HEK293 Cells in Monolayer and Spheroids

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Science, Lavrent’ev av., 8, 630090 Novosibirsk, Russia
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Nanomaterials 2020, 10(10), 2040; https://doi.org/10.3390/nano10102040
Received: 27 August 2020 / Revised: 13 October 2020 / Accepted: 13 October 2020 / Published: 16 October 2020
(This article belongs to the Special Issue Advances in Nanomaterials in Biomedicine)
Use of multicellular spheroids in studies of nanoparticles (NPs) has increased in the last decade, however details of NPs interaction with spheroids are poorly known. We synthesized AuNPs (12.0 ± 0.1 nm in diameter, transmission electron microscopy (TEM data) and covered them with bovine serum albumin (BSA) and polyethyleneimine (PEI). Values of hydrodynamic diameter were 17.4 ± 0.4; 35.9 ± 0.5 and ±125.9 ± 2.8 nm for AuNPs, AuBSA-NPs and AuPEI-NPs, and Z-potential (net charge) values were −33.6 ± 2.0; −35.7 ± 1.8 and 39.9 ± 1.3 mV, respectively. Spheroids of human hepatocarcinoma (HepG2) and human embryo kidney (HEK293) cells (Corning ® spheroid microplates CLS4515-5EA), and monolayers of these cell lines were incubated with all NPs for 15 min–4 h, and fixed in 4% paraformaldehyde solution. Samples were examined using transmission and scanning electron microscopy. HepG2 and HEK2893 spheroids showed tissue-specific features and contacted with culture medium by basal plasma membrane of the cells. HepG2 cells both in monolayer and spheroids did not uptake of the AuNPs, while AuBSA-NPs and AuPEI-NPs readily penetrated these cells. All studied NPs penetrated HEK293 cells in both monolayer and spheroids. Thus, two different cell cultures maintained a type of the interaction with NPs in monolayer and spheroid forms, which not depended on NPs Z-potential and size. View Full-Text
Keywords: AuNPs; AuPEI-NPs; AuBSA-NPs; electron microscopy; ultrastructure of HepG2 cells and spheroids; ultrastructure of HEK293 cells and spheroids; penetration of NPs into monolayer and spheroids AuNPs; AuPEI-NPs; AuBSA-NPs; electron microscopy; ultrastructure of HepG2 cells and spheroids; ultrastructure of HEK293 cells and spheroids; penetration of NPs into monolayer and spheroids
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MDPI and ACS Style

Chelobanov, B.; Poletaeva, J.; Epanchintseva, A.; Tupitsyna, A.; Pyshnaya, I.; Ryabchikova, E. Ultrastructural Features of Gold Nanoparticles Interaction with HepG2 and HEK293 Cells in Monolayer and Spheroids. Nanomaterials 2020, 10, 2040.

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