Effects of Zn-Doped Mesoporous Bioactive Glass Nanoparticles in Etch-and-Rinse Adhesive on the Microtensile Bond Strength
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis of MBN and MBN-Zn
2.2. Characterisation of MBN and MBN-Zn
2.3. Fabrication of Experimental Samples
2.3.1. Preparation of DA Mixed with MBN (DA-MBN) and DA Mixed with Zn-Doped MBN (DA-MBN-Zn)
2.3.2. Resin Disk Preparation
2.3.3. Resin/Dentin Beam Preparation
2.4. Dissolution Test
2.5. Matrix Metalloproteinases (MMPs) Inhibition Test
2.6. Mechanical Properties
2.6.1. Microtensile Bond Strength (MTBS) Test
2.6.2. Thermocycling Test
2.7. Biological Properties
2.7.1. Cell Culture and MTT Assay
2.7.2. Quantitative Assay of Alkaline Phosphatase (ALP) Activity
2.7.3. Alizarin Red S (ARS) Staining and Quantitative Detection
2.8. Non-Cellular Bioactivity of DA-MBN and DA-MBN-Zn
2.9. Statistical Analysis
3. Results
3.1. Characterisation of MBN and MBN-Zn
3.2. Characterisation of DA-MBN and DA-MBN-Zn
In-Vitro Dissolution Test
3.3. MMP Inhibition Test
3.4. Mechanical Properties
3.4.1. MTBS Test
3.4.2. MTBS Test after Thermocycling
3.5. Biological Properties
3.5.1. MTT Assay
3.5.2. ALP Activity in hDPSCs
3.5.3. ARS Staining
3.6. Non-Cellular Bioactivity of DA-MBN and DA-MBN-Zn
3.6.1. SEM after SBF Soaking
3.6.2. XRD after SBF Soaking
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Composition (mol%) | ||||
---|---|---|---|---|
SiO2 | CaO | P2O5 | ZnO | |
MBN | 65 | 31 | 4 | 0 |
MBN-Zn | 60 | 31 | 4 | 5 |
Group | n | MTBS | MTBS after Thermocycling |
---|---|---|---|
DA | 15 | 36.59 ± 10.66 | 28.77 ± 4.16 |
DA-0.1% MBN | 15 | 36.83 ± 10.18 | 35.66 ± 4.35 |
DA-0.5% MBN | 15 | 37.59 ± 11.31 | 36.98 ± 5.30 |
DA-1.0% MBN | 15 | 37.64 ± 8.11 | 37.57 ± 10.29 * |
DA-0.1% MBN-Zn | 15 | 36.25 ± 7.50 | 35.59 ± 10.02 |
DA-0.5% MBN-Zn | 15 | 37.02 ± 8.89 | 35.81 ± 11.35 |
DA-1.0% MBN-Zn | 15 | 38.39 ± 8.09 | 37.85 ± 7.12 * |
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Choi, Y.; Sun, W.; Kim, Y.; Kim, I.-R.; Gong, M.-K.; Yoon, S.-Y.; Bae, M.-K.; Park, B.-S.; Park, S.-B.; Kim, Y.-I. Effects of Zn-Doped Mesoporous Bioactive Glass Nanoparticles in Etch-and-Rinse Adhesive on the Microtensile Bond Strength. Nanomaterials 2020, 10, 1943. https://doi.org/10.3390/nano10101943
Choi Y, Sun W, Kim Y, Kim I-R, Gong M-K, Yoon S-Y, Bae M-K, Park B-S, Park S-B, Kim Y-I. Effects of Zn-Doped Mesoporous Bioactive Glass Nanoparticles in Etch-and-Rinse Adhesive on the Microtensile Bond Strength. Nanomaterials. 2020; 10(10):1943. https://doi.org/10.3390/nano10101943
Chicago/Turabian StyleChoi, Yeonju, Woogyeong Sun, Yeon Kim, In-Ryoung Kim, Mi-Kyung Gong, Seog-Young Yoon, Moon-Kyoung Bae, Bong-Soo Park, Soo-Byung Park, and Yong-Il Kim. 2020. "Effects of Zn-Doped Mesoporous Bioactive Glass Nanoparticles in Etch-and-Rinse Adhesive on the Microtensile Bond Strength" Nanomaterials 10, no. 10: 1943. https://doi.org/10.3390/nano10101943