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Open AccessArticle

Silica-Coated Magnetic Iron Oxide Nanoparticles Grafted onto Graphene Oxide for Protein Isolation

1
Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea
2
Laboratory of Biofuel and Biomass Research, VNU-HCMU University of Technology, 268 Ly Thuong Kiet street, district 10, Ho Chi Minh 700000, Vietnam
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(1), 117; https://doi.org/10.3390/nano10010117
Received: 28 November 2019 / Revised: 4 January 2020 / Accepted: 6 January 2020 / Published: 8 January 2020
(This article belongs to the Special Issue Nano-hybrids: Synthesis, Characterization and Applications)
In this study, silica-coated magnetic iron oxide nanoparticles ([email protected]2) were covalently conjugated onto graphene oxide (GO/[email protected]2) for protein isolation. First, MNPs were precisely coated with a silica layer on the surface by using the reverse microemulsion method, followed by incubation with 3-glycidyloxypropyltrimethoxysilane (GPTS) to produce the GPTS-functionalized [email protected]2 (GPTS-coated [email protected]2) that display epoxy groups on the surface. The silica shell on the MNPs was optimized at 300 µL of Igepal®CO-520, 5 mg of MNP, 100 µL of TEOS, 100 µL of NH4OH and 3% of 3-glycidyloxypropyltrimethoxysilane (GPTS). Simultaneously, polyethyleneimine (PEI) was covalently conjugated to GO to enhance the stability of GO in aqueous solutions and create the reaction sites with epoxy groups on the surface of GPTS-coated [email protected]2. The ratio of PEI grafted GO and GPTS-coated [email protected]2 (GO/MNP ratio) was investigated to produce GO/[email protected]2 with highly saturated magnetization without aggregation. As a result, the GO/MNP ratio of 5 was the best condition to produce the GO/[email protected]2 with 9.53 emu/g of saturation superparamagnetization at a magnetic field of 2.0 (T). Finally, the GO/[email protected]2 were used to separate bovine serum albumin (BSA) to investigate its protein isolation ability. The quantity of BSA adsorbed onto 1 mg of GO/[email protected]2 increased sharply over time to reach 628 ± 9.3 µg/mg after 15 min, which was 3.5-fold-higher than that of GPTS-coated [email protected]2. This result suggests that the GO/[email protected]2 nanostructure can be used for protein isolation. View Full-Text
Keywords: graphene oxide; superparamagnetic iron oxide nanoparticles; reverse microemulsion; silica-coated superparamagnetic nanoparticle; superparamagnetic nanoparticle embedded graphene oxide; protein isolation graphene oxide; superparamagnetic iron oxide nanoparticles; reverse microemulsion; silica-coated superparamagnetic nanoparticle; superparamagnetic nanoparticle embedded graphene oxide; protein isolation
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Pham, X.-H.; Hahm, E.; Kim, H.-M.; Son, B.S.; Jo, A.; An, J.; Tran Thi, T.A.; Nguyen, D.Q.; Jun, B.-H. Silica-Coated Magnetic Iron Oxide Nanoparticles Grafted onto Graphene Oxide for Protein Isolation. Nanomaterials 2020, 10, 117.

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