Molecular Characterization and Expression Analysis of Novel Interleukin-1 Family Member (nIL-1Fm) Gene in Nile Tilapia (Oreochromis niloticus)
Abstract
:1. Introduction
2. Materials and Methods
2.1. The Experimental Fish
2.2. Identification and Cloning of nIL-1Fm
2.3. Bioinformatics Analysis of nIL-1Fm
2.4. Tissue Distribution of nIL-1Fm by Quantitative Real-Time PCR (qRT-PCR)
2.5. Modulation of the Expression of Nile Tilapia nIL-1Fm In Vivo by Bacterial S. agalactiae Infection
2.6. Modulation of nIL-1Fm Expression in PBL by PAMPs
2.7. Statistical Analysis
3. Results
3.1. Sequence Analysis of the nIL-1Fm Gene
3.2. Multiple Sequence Alignment and Synteny Analysis
3.3. Phylogenetic Analysis of nIL-1Fm
3.4. Gene Organization of Nile Tilapia nIL-1Fm
3.5. Tissue Distribution
3.6. Modulation of IL-1β and nIL-1Fm Expression to PAMPs in PBLs
3.7. Expression Profiles of IL-1β and nIL-1Fm in Response to LPS
3.8. Expression of IL-1β and nIL-1Fm under S. agalactiae Challenge
4. Discussion
5. Conclusions
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Primer Name | Primer Sequence (5′–3′) | Application |
---|---|---|---|
nIL-1Fm | Full IL-1X F | ATGGACACGAAGGAGTCTCTGGT | Cloning |
Full IL-1X R | TCACTTTTGAATGATGAAGAGGAA | Cloning | |
IL-1X F | GGAGGATGACCCGGAGGGAA | qRT-PCR | |
IL-1X R | ATCACAGCCCCTGCCTCTCA | qRT-PCR | |
IL-1β | IL-1B F | AAGATGAATTGTGGAGCTGTGTT | qRT-PCR |
IL-1B R | AAAAGCATCGACAGTATGTGAAAT | qRT-PCR | |
β-actin | β-actin F | ACAGGATGCAGAAGGAGATCACAG | qRT-PCR |
β-actin R | GTACTCCTGCTTGCTGATCCACAT | qRT-PCR |
Species Name | GenBank ID | Length (bp) | ORF (AA) | Mw (kDa)/pI | Similarity (%) | Identity (%) |
---|---|---|---|---|---|---|
Mammalian IL-1Ra | ||||||
Human | CAA36262 | 1740 | 177 | 20.05/5.82 | 17.7 | 11.7 |
Rhesus monkey | XP_001091833 | 1723 | 177 | 19.89/6.89 | 19.6 | 12.9 |
Bird IL-1Ra | ||||||
Chicken | CCD83119 | 554 | 173 | 19.37/8.68 | 19.7 | 9.3 |
Duck | XP_032060067 | 515 | 162 | 18.17/7.74 | 22.1 | 11.6 |
Fish nIL-1Fm | ||||||
Nile tilapia | ON568309 | 2474 | 350 | 39.12/4.97 | - | - |
Common carp | XM_042734003 | 1766 | 353 | 40.40/5.22 | 47.8 | 32.6 |
Fathead minnow | XP_039529760 | 2016 | 347 | 39.07/5.13 | 49.1 | 33.4 |
Grass carp | KM066966 | 1260 | 347 | 39.48/4.96 | 49.1 | 33.4 |
Zebrafish | NP_001277347 | 1071 | 356 | 39.55/5.17 | 49.6 | 32.6 |
Channel catfish | XP_017314635 | 2034 | 354 | 39.35/5.45 | 51.7 | 35.7 |
Rainbow trout | AJ555869 | 1736 | 365 | 41.18/5.67 | 60.0 | 45.4 |
Medaka | XM004084753 | 1380 | 348 | 39.57/7.53 | 65.1 | 47.4 |
Fugu | HF936683 | 1122 | 373 | 42.27/5.21 | 67.8 | 50.7 |
Flounder | AB720987 | 1083 | 354 | 39.83/5.15 | 68.8 | 50.4 |
Asian seabass | XP_018539191 | 2161 | 354 | 39.77/5.59 | 69.8 | 55.4 |
Gilthead seabream | XP_030271237 | 1879 | 359 | 39.82/5.63 | 73.1 | 57.3 |
Yellow croaker | XP_010730145 | 1785 | 360 | 39.97/5.30 | 76.1 | 61.3 |
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Wangkahart, E.; Jumpalueang, S.; Ardprachan, S.; Phudkliang, J.; Sunthamala, P.; Pholchamat, S.; Qi, Z. Molecular Characterization and Expression Analysis of Novel Interleukin-1 Family Member (nIL-1Fm) Gene in Nile Tilapia (Oreochromis niloticus). J. Mar. Sci. Eng. 2022, 10, 1272. https://doi.org/10.3390/jmse10091272
Wangkahart E, Jumpalueang S, Ardprachan S, Phudkliang J, Sunthamala P, Pholchamat S, Qi Z. Molecular Characterization and Expression Analysis of Novel Interleukin-1 Family Member (nIL-1Fm) Gene in Nile Tilapia (Oreochromis niloticus). Journal of Marine Science and Engineering. 2022; 10(9):1272. https://doi.org/10.3390/jmse10091272
Chicago/Turabian StyleWangkahart, Eakapol, Sukanda Jumpalueang, Supanniga Ardprachan, Janjira Phudkliang, Phitcharat Sunthamala, Sirinya Pholchamat, and Zhitao Qi. 2022. "Molecular Characterization and Expression Analysis of Novel Interleukin-1 Family Member (nIL-1Fm) Gene in Nile Tilapia (Oreochromis niloticus)" Journal of Marine Science and Engineering 10, no. 9: 1272. https://doi.org/10.3390/jmse10091272
APA StyleWangkahart, E., Jumpalueang, S., Ardprachan, S., Phudkliang, J., Sunthamala, P., Pholchamat, S., & Qi, Z. (2022). Molecular Characterization and Expression Analysis of Novel Interleukin-1 Family Member (nIL-1Fm) Gene in Nile Tilapia (Oreochromis niloticus). Journal of Marine Science and Engineering, 10(9), 1272. https://doi.org/10.3390/jmse10091272