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Article

Detection of Circulating Tumor Cells in Renal Cell Carcinoma: Disease Stage Correlation and Molecular Characterization

1
Department of Urology, University Hospital Kralovske Vinohrady, 3rd Faculty of Medicine Charles University in Prague, Srobarova 50, 10034 Prague, Czech Republic
2
Department of Laboratory Genetics, University Hospital Kralovske Vinohrady, Srobarova 50, 10034 Prague, Czech Republic
3
Department of Thoracic Surgery, Lower Silesian Cancer Center, Wroclaw, Plac Ludwika Hirszfelda 12, 53-413 Wrocław, Poland
4
3rd Department of Surgery University Hospital Motol, 1st Faculty of Medicine Charles University, V Uvalu 84, 150 06 Prague, Czech Republic
5
Department of Histology and Embryology, Wroclaw Medical University, Wybrzeże Ludwika Pasteura 1, 50-367 Wrocław, Poland
6
Department of Thoracic Surgery, Masaryk´s Hospital, Krajska zdravotni a.s., Socialni pece 3316/12A, 40113 Usti nad Labem, Czech Republic
*
Author to whom correspondence should be addressed.
J. Clin. Med. 2020, 9(5), 1372; https://doi.org/10.3390/jcm9051372
Received: 22 March 2020 / Revised: 27 April 2020 / Accepted: 28 April 2020 / Published: 7 May 2020
(This article belongs to the Special Issue Circulating Biomarkers as a Liquid Biopsy for Cancer)
The presence of circulating tumor cells (CTCs) in patients with solid tumors is associated with poor prognosis. However, there are limited data concerning the detection of CTCs in renal cell cancer (RCC). The aim of this study is to evaluate the presence of CTCs in peripheral blood of patients with RCC undergoing surgery (n = 186). CTCs were tested before and after surgery as well as during the follow-up period afterwards. In total 495 CTC testing in duplicates were provided. To enrich CTCs, a size-based separation protocol and tube MetaCell® was used. CTCs presence was evaluated by single cell cytomorphology based on vital fluorescence microscopy. Additionally, to standardly applied fluorescence stains, CTCs viability was controlled by mitochondrial activity. CTCs were detected independently on the sampling order in up to 86.7% of the tested blood samples in patients undergoing RCC surgery. There is higher probability of CTC detection with growing tumor size, especially in clear cell renal cell cancer (ccRCC) cases. Similarly, the tumor size corresponds with metastasis presence and lymph node positivity and CTC detection. This paper describes for the first-time successful analysis of viable CTCs and their mitochondria as a part of the functional characterization of CTCs in RCC. View Full-Text
Keywords: CTCs; renal cancer; culturing; in vitro; MetaCell; gene expression; immunotherapy; PDL-1 CTCs; renal cancer; culturing; in vitro; MetaCell; gene expression; immunotherapy; PDL-1
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MDPI and ACS Style

Klezl, P.; Pospisilova, E.; Kolostova, K.; Sonsky, J.; Maly, O.; Grill, R.; Pawlak, I.; Bobek, V. Detection of Circulating Tumor Cells in Renal Cell Carcinoma: Disease Stage Correlation and Molecular Characterization. J. Clin. Med. 2020, 9, 1372. https://doi.org/10.3390/jcm9051372

AMA Style

Klezl P, Pospisilova E, Kolostova K, Sonsky J, Maly O, Grill R, Pawlak I, Bobek V. Detection of Circulating Tumor Cells in Renal Cell Carcinoma: Disease Stage Correlation and Molecular Characterization. Journal of Clinical Medicine. 2020; 9(5):1372. https://doi.org/10.3390/jcm9051372

Chicago/Turabian Style

Klezl, Petr, Eliska Pospisilova, Katarina Kolostova, Jindrich Sonsky, Ondrej Maly, Robert Grill, Ireneusz Pawlak, and Vladimir Bobek. 2020. "Detection of Circulating Tumor Cells in Renal Cell Carcinoma: Disease Stage Correlation and Molecular Characterization" Journal of Clinical Medicine 9, no. 5: 1372. https://doi.org/10.3390/jcm9051372

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