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Open AccessArticle

Rapid and Efficient Isolation of Exosomes by Clustering and Scattering

by Jinhyun Kim 1,†, Hoyoon Lee 1,†, KyongHwa Park 2,3 and Sehyun Shin 1,3,*
School of Mechanical engineering, Korea University, Seoul 02841, Korea
Department of Internal Medicine, Anam Hospital, Korea University, Seoul 02841, Korea
Engineering Research Center for Biofluid Biopsy, Seoul 02841, Korea
Author to whom correspondence should be addressed.
Jinhyun Kim and Hoyoon Lee contributed equally to the article as first authors.
J. Clin. Med. 2020, 9(3), 650;
Received: 6 February 2020 / Revised: 21 February 2020 / Accepted: 25 February 2020 / Published: 28 February 2020
(This article belongs to the Special Issue Circulating Biomarkers as a Liquid Biopsy for Cancer)
Tumor-derived extracellular vesicles (EVs) have become important biomarkers of liquid biopsies for precision medicine. However, the clinical application of EVs has been limited due to the lack of EV isolation practical technology applicable to clinical environments. Here, we report an innovative EV isolation method, which is quick and simple, and facilitates high-yield and high-purity EV isolation from blood. Introducing a cationic polymer in plasma resulted in rapid clustering of anionic EVs and a chaotropic agent can separate EVs from these clusters. Isolated EVs were characterized in terms of size distribution, morphology, surface protein markers, and exosomal RNA. Through performance comparison with various methods, including ultracentrifugation (UC), the present method delivered the highest recovery rate (~20 folds that of UC) and purity ratio (3.5 folds that of UC) of EVs in a short period of time (<20 min). The proposed method is expected to be used in basic and applied research on EV isolation and in clinical applications. View Full-Text
Keywords: tumor-derive extracellular vesicles; isolation; liquid biopsy; blood; polymer tumor-derive extracellular vesicles; isolation; liquid biopsy; blood; polymer
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MDPI and ACS Style

Kim, J.; Lee, H.; Park, K.; Shin, S. Rapid and Efficient Isolation of Exosomes by Clustering and Scattering. J. Clin. Med. 2020, 9, 650.

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