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Article

Diagnostic Utility and Pathogenic Role of Circulating MicroRNAs in Vasospastic Angina

1
Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea
2
Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 03080, Korea
3
Department of Internal Medicine, Seoul National University Hospital, Seoul 03080, Korea
4
Department of Internal Medicine, Seoul National University College of Medicine; Seoul 03080, Korea
5
Department of Internal Medicine, Seoul Metropolitan Government Seoul National University Boramae Medical Center, Seoul 07061, Korea
*
Author to whom correspondence should be addressed.
These authors contribution is equally to this work.
J. Clin. Med. 2020, 9(5), 1313; https://doi.org/10.3390/jcm9051313
Received: 2 April 2020 / Revised: 26 April 2020 / Accepted: 27 April 2020 / Published: 2 May 2020
(This article belongs to the Section Cardiology)
We investigated the diagnostic value and pathophysiological role of circulating microRNA (miR) in vasospastic angina (VA). We enrolled patients who underwent coronary angiography for chest pain to explore the miR’s diagnostic utility. In addition, we investigated the role of miRs in regulating endothelial nitric oxide synthase (eNOS) expression in human coronary artery endothelial cells (hCAECs). Among the 121 patients, 46 were diagnosed with VA (VA group), 26 with insignificant coronary lesions (ICL group), and 49 with atherothrombotic angina (AA group). The VA group showed a significantly higher expression of miR-17-5p, miR-92a-3p, and miR-126-3p than the ICL group. In contrast, miR-221-3p and miR-222-3p were upregulated in the AA group compared to the VA group, and all levels of miR-17-5p, miR-92a-3p, miR-126-3p, miR-145-5p, miR-221-3p, and miR-222-3p differed between the AA group and the ICL group. In the hCAECs, transfection with mimics (pre-miR) of miR-17-5p, miR-92a-3p, and miR-126-3p was associated with eNOS suppression. Additionally, transfection with inhibitors (anti-miR) of miR-92a-3p significantly rescued the eNOS suppression induced by lipopolysaccharide. In conclusion, the circulating miRs not only proved to have diagnostic utility, but also contributed to pathogenesis by eNOS regulation. View Full-Text
Keywords: vasospastic angina; microRNA; diagnosis; pathogenesis vasospastic angina; microRNA; diagnosis; pathogenesis
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MDPI and ACS Style

Park, C.S.; Kim, I.; Oh, G.C.; Han, J.-K.; Yang, H.-M.; Park, K.W.; Cho, H.-J.; Kang, H.-J.; Koo, B.-K.; Chung, W.-Y.; Oh, S.; Lee, H.-Y. Diagnostic Utility and Pathogenic Role of Circulating MicroRNAs in Vasospastic Angina. J. Clin. Med. 2020, 9, 1313. https://doi.org/10.3390/jcm9051313

AMA Style

Park CS, Kim I, Oh GC, Han J-K, Yang H-M, Park KW, Cho H-J, Kang H-J, Koo B-K, Chung W-Y, Oh S, Lee H-Y. Diagnostic Utility and Pathogenic Role of Circulating MicroRNAs in Vasospastic Angina. Journal of Clinical Medicine. 2020; 9(5):1313. https://doi.org/10.3390/jcm9051313

Chicago/Turabian Style

Park, Chan S., Inho Kim, Gyu C. Oh, Jung-Kyu Han, Han-Mo Yang, Kyung W. Park, Hyun-Jai Cho, Hyun-Jae Kang, Bon-Kwon Koo, Woo-Young Chung, Seil Oh, and Hae-Young Lee. 2020. "Diagnostic Utility and Pathogenic Role of Circulating MicroRNAs in Vasospastic Angina" Journal of Clinical Medicine 9, no. 5: 1313. https://doi.org/10.3390/jcm9051313

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