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Open AccessArticle

An Original ELISA-Based Multiplex Method for the Simultaneous Detection of 5 SARS-CoV-2 IgG Antibodies Directed against Different Antigens

1
Department of Pharmacy, Namur Research Institute for Life Sciences, University of Namur, B-5000 Namur, Belgium
2
Central Laboratory Department, Qualiblood sa, 5000 Namur, Belgium
3
Department of Laboratory Medicine, Clinique St-Luc Bouge, 5004 Namur, Belgium
4
Department of Laboratory Medicine, Iris Hospitals South, 1060 Brussels, Belgium
5
Faculty of Medicine, Université libre de Bruxelles, 1050 Brussels, Belgium
*
Author to whom correspondence should be addressed.
J. Clin. Med. 2020, 9(11), 3752; https://doi.org/10.3390/jcm9113752
Received: 25 October 2020 / Revised: 17 November 2020 / Accepted: 19 November 2020 / Published: 21 November 2020
(This article belongs to the Section Molecular Diagnostics)
Strategies to detect SARS-CoV-2 are increasingly being developed. Among them, serological methods have been developed. Nevertheless, although these may present an interesting clinical performance, they are often directed against only one antigen. This study aims at evaluating the clinical performance of an innovative multiplex immunoassay (i.e., CoViDiag assay) detecting simultaneously the presence of antibodies directed against N, S1, S2, RBD and NTD antigens. Sensitivity was evaluated in 135 samples obtained from 94 rRT-PCR confirmed coronavirus disease 2019 (COVID-19) patients. Non-SARS-CoV-2 sera (n = 132) collected before the COVID-19 pandemic with potential cross-reactions to the SARS-CoV-2 immunoassay were included in the specificity analysis. The antibody signature was also studied in hospitalized and non-hospitalized patients. The specificity of the CoViDiag assay was excellent for all antibodies (99.2 to 100%) using adapted cut-offs. None of the false positive samples were positive for more than one antibody. The sensitivity obtained from samples collected 14 days since symptom onset varied from 92.0 to 100.0% depending on the antibody considered. Among samples collected more than 14 days after symptom onset, 12.8, 66.3, 3.5, 9.3, 5.8 and 2.3% were positive for 5, 4, 3, 2, 1 or 0 antibodies, respectively. A trend toward higher antibody titers was observed in hospitalized patient in the early days since symptom onset. However, no significant difference was observed compared to non-hospitalized patients after 14 days since symptom onset. The clinical performance of the CoViDiag 5 IgG assay is sufficient to recommend its use for the detection and the characterization of the antibody signature following SARS-CoV-2 infection. The combination of several antigens in the same test improves the overall specificity and sensitivity of the test. Further research is needed to investigate whether this strategy may be of interest to identify severe disease outcome in patients with SARS-CoV-2 infection. View Full-Text
Keywords: COVID-19; SARS-CoV-2; multiplex; serology; kinetics COVID-19; SARS-CoV-2; multiplex; serology; kinetics
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MDPI and ACS Style

Gillot, C.; Douxfils, J.; Cadrobbi, J.; Laffineur, K.; Dogné, J.-M.; Elsen, M.; Eucher, C.; Melchionda, S.; Modaffarri, É.; Tré-Hardy, M.; Favresse, J. An Original ELISA-Based Multiplex Method for the Simultaneous Detection of 5 SARS-CoV-2 IgG Antibodies Directed against Different Antigens. J. Clin. Med. 2020, 9, 3752. https://doi.org/10.3390/jcm9113752

AMA Style

Gillot C, Douxfils J, Cadrobbi J, Laffineur K, Dogné J-M, Elsen M, Eucher C, Melchionda S, Modaffarri É, Tré-Hardy M, Favresse J. An Original ELISA-Based Multiplex Method for the Simultaneous Detection of 5 SARS-CoV-2 IgG Antibodies Directed against Different Antigens. Journal of Clinical Medicine. 2020; 9(11):3752. https://doi.org/10.3390/jcm9113752

Chicago/Turabian Style

Gillot, Constant; Douxfils, Jonathan; Cadrobbi, Julie; Laffineur, Kim; Dogné, Jean-Michel; Elsen, Marc; Eucher, Christine; Melchionda, Sabrina; Modaffarri, Élise; Tré-Hardy, Marie; Favresse, Julien. 2020. "An Original ELISA-Based Multiplex Method for the Simultaneous Detection of 5 SARS-CoV-2 IgG Antibodies Directed against Different Antigens" J. Clin. Med. 9, no. 11: 3752. https://doi.org/10.3390/jcm9113752

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