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Open AccessArticle

Expression of Taste Receptor 2 Subtypes in Human Testis and Sperm

1
Department of Molecular and Developmental Medicine, Siena University, 53100 Siena, Italy
2
Walther Straub Institute of Pharmacology and Toxicology, LMU Munich, 80336 Muenchen, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
J. Clin. Med. 2020, 9(1), 264; https://doi.org/10.3390/jcm9010264
Received: 13 December 2019 / Revised: 10 January 2020 / Accepted: 14 January 2020 / Published: 18 January 2020
(This article belongs to the Special Issue Approach to Male Infertility and Induction of Spermatogenesis)
Taste receptors (TASRs) are expressed not only in the oral cavity but also throughout the body, thus suggesting that they may play different roles in organ systems beyond the tongue. Recent studies showed the expression of several TASRs in mammalian testis and sperm, indicating an involvement of these receptors in male gametogenesis and fertility. This notion is supported by an impaired reproductive phenotype of mouse carrying targeted deletion of taste receptor genes, as well as by a significant correlation between human semen parameters and specific polymorphisms of taste receptor genes. To better understand the biological and thus clinical significance of these receptors for human reproduction, we analyzed the expression of several members of the TAS2Rs family of bitter receptors in human testis and in ejaculated sperm before and after in vitro selection and capacitation. Our results provide evidence for the expression of TAS2R genes, with TAS2R14 being the most expressed bitter receptor subtype in both testis tissue and sperm cells, respectively. In addition, it was observed that in vitro capacitation significantly affects both the expression and the subcellular localization of these receptors in isolated spermatozoa. Interestingly, α-gustducin and α-transducin, two Gα subunits expressed in taste buds on the tongue, are also expressed in human spermatozoa; moreover, a subcellular redistribution of both G protein α-subunits to different sub-compartments of sperm was registered upon in vitro capacitation. Finally, we shed light on the possible downstream transduction pathway initiated upon taste receptor activation in the male reproductive system. Performing ultrasensitive droplets digital PCR assays to quantify RNA copy numbers of a distinct gene, we found a significant correlation between the expression of TAS2Rs and TRPM5 (r = 0.87), the cation channel involved in bitter but also sweet and umami taste transduction in taste buds on the tongue. Even if further studies are needed to clarify the precise functional role of taste receptors for successful reproduction, the presented findings significantly extend our knowledge of the biological role of TAS2Rs for human male fertility. View Full-Text
Keywords: human male fertility; bitter taste receptors; human sperm; human testis; GPCRs; α-gustducin; α-transducin; TRPM5 human male fertility; bitter taste receptors; human sperm; human testis; GPCRs; α-gustducin; α-transducin; TRPM5
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MDPI and ACS Style

Governini, L.; Semplici, B.; Pavone, V.; Crifasi, L.; Marrocco, C.; De Leo, V.; Arlt, E.; Gudermann, T.; Boekhoff, I.; Luddi, A.; Piomboni, P. Expression of Taste Receptor 2 Subtypes in Human Testis and Sperm. J. Clin. Med. 2020, 9, 264. https://doi.org/10.3390/jcm9010264

AMA Style

Governini L, Semplici B, Pavone V, Crifasi L, Marrocco C, De Leo V, Arlt E, Gudermann T, Boekhoff I, Luddi A, Piomboni P. Expression of Taste Receptor 2 Subtypes in Human Testis and Sperm. Journal of Clinical Medicine. 2020; 9(1):264. https://doi.org/10.3390/jcm9010264

Chicago/Turabian Style

Governini, Laura; Semplici, Bianca; Pavone, Valentina; Crifasi, Laura; Marrocco, Camilla; De Leo, Vincenzo; Arlt, Elisabeth; Gudermann, Thomas; Boekhoff, Ingrid; Luddi, Alice; Piomboni, Paola. 2020. "Expression of Taste Receptor 2 Subtypes in Human Testis and Sperm" J. Clin. Med. 9, no. 1: 264. https://doi.org/10.3390/jcm9010264

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