Next Article in Journal
Patient-Derived Colorectal Cancer Organoids Upregulate Revival Stem Cell Marker Genes following Chemotherapeutic Treatment
Previous Article in Journal
Back to The Fusion: Mitofusin-2 in Alzheimer’s Disease
Previous Article in Special Issue
Erastin Inhibits Septic Shock and Inflammatory Gene Expression via Suppression of the NF-κB Pathway
Open AccessArticle

Differential Gene Expression in Circulating CD14+ Monocytes Indicates the Prognosis of Critically Ill Patients with Sepsis

1
Department of Medicine III, University Hospital Aachen, RWTH Aachen University, 52074 Aachen, Germany
2
Department of Anaesthesiology, University Hospital Aachen, RWTH Aachen University, 52074 Aachen, Germany
3
Genomics and Immunoregulation, Life & Medical Sciences (LIMES) Institute, University of Bonn, 53115 Bonn, Germany
4
Department of Internal Medicine and Radboud Center for Infectious Diseases (RCI), Radboud University Medical Center, 6525 Nijmegen, The Netherlands
5
Department of Hepatology & Gastroenterology, Campus Virchow Klinikum and Charité Campus Mitte, Charité University Medicine Berlin, 13353 Berlin, Germany
*
Author to whom correspondence should be addressed.
J. Clin. Med. 2020, 9(1), 127; https://doi.org/10.3390/jcm9010127
Received: 25 November 2019 / Revised: 30 December 2019 / Accepted: 31 December 2019 / Published: 2 January 2020
Critical illness and sepsis are characterized by drastic changes in the systemic innate immune response, particularly involving monocytes. The exact monocyte activation profile during sepsis, however, has remained obscure. Therefore, we prospectively analyzed the gene expression profile of circulating CD14+ monocytes from healthy volunteers (n = 54) and intensive care unit (ICU) patients (n = 76), of which n = 36 had sepsis. RNA sequencing of selected samples revealed that monocytes from septic ICU patients display a peculiar activation pattern, which resembles characteristic functional stages of monocyte-derived macrophages and is distinct from controls or non-sepsis ICU patients. Focusing on 55 highly variable genes selected for further investigation, arachidonate 5-lipoxygenase-activating protein (ALOX5AP) was highly upregulated in monocytes of ICU patients and only normalized during 7 days in the ICU in non-sepsis patients. Strikingly, low monocytic guanine nucleotide exchange factor 10-like protein (ARHGEF10L) mRNA expression was associated with the disease severity and mortality of ICU patients. Collectively, our comprehensive analysis of circulating monocytes in critically ill patients revealed a distinct activation pattern, particularly in ICU patients with sepsis. The association with disease severity, the longitudinal recovery or lack thereof during the ICU stay, and the association with prognosis indicate the clinical relevance of monocytic gene expression profiles during sepsis. View Full-Text
Keywords: ICU; sepsis; mortality; prognosis; innate immunity; monocytes; biomarker ICU; sepsis; mortality; prognosis; innate immunity; monocytes; biomarker
Show Figures

Figure 1

MDPI and ACS Style

Liepelt, A.; Hohlstein, P.; Gussen, H.; Xue, J.; Aschenbrenner, A.C.; Ulas, T.; Buendgens, L.; Warzecha, K.T.; Bartneck, M.; Luedde, T.; Trautwein, C.; Schultze, J.L.; Koch, A.; Tacke, F. Differential Gene Expression in Circulating CD14+ Monocytes Indicates the Prognosis of Critically Ill Patients with Sepsis. J. Clin. Med. 2020, 9, 127.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop