Systemic Sclerosis Serum Steers the Differentiation of Adipose-Derived Stem Cells Toward Profibrotic Myofibroblasts: Pathophysiologic Implications
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients and Serum Samples
2.2. Culture of Human ADSC
2.3. In Vitro Differentiation of Human ADSC into Mature Adipocytes
2.4. Oil Red O Staining
2.5. Stimulation of Human ADSC to Assess Myofibroblastic Differentiation
2.6. RNA Isolation and Quantitative Real-Time PCR
2.7. Fluorescence Immunocytochemistry
2.8. Immunoblotting
2.9. Collagen Gel Contraction Assay
2.10. Determination of TGFβ1 Serum Levels
2.11. Statistical Analysis
3. Results
3.1. Treatment with SSc Serum Significantly Inhibits the Adipogenic Differentiation Process of Normal Human ADSC
3.2. Treatment with SSc Serum Induces a Myofibroblast-Like Profibrotic and Contractile Phenotype in Normal Human ADSC
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Manetti, M.; Romano, E.; Rosa, I.; Fioretto, B.S.; Praino, E.; Guiducci, S.; Iannone, F.; Ibba-Manneschi, L.; Matucci-Cerinic, M. Systemic Sclerosis Serum Steers the Differentiation of Adipose-Derived Stem Cells Toward Profibrotic Myofibroblasts: Pathophysiologic Implications. J. Clin. Med. 2019, 8, 1256. https://doi.org/10.3390/jcm8081256
Manetti M, Romano E, Rosa I, Fioretto BS, Praino E, Guiducci S, Iannone F, Ibba-Manneschi L, Matucci-Cerinic M. Systemic Sclerosis Serum Steers the Differentiation of Adipose-Derived Stem Cells Toward Profibrotic Myofibroblasts: Pathophysiologic Implications. Journal of Clinical Medicine. 2019; 8(8):1256. https://doi.org/10.3390/jcm8081256
Chicago/Turabian StyleManetti, Mirko, Eloisa Romano, Irene Rosa, Bianca Saveria Fioretto, Emanuela Praino, Serena Guiducci, Florenzo Iannone, Lidia Ibba-Manneschi, and Marco Matucci-Cerinic. 2019. "Systemic Sclerosis Serum Steers the Differentiation of Adipose-Derived Stem Cells Toward Profibrotic Myofibroblasts: Pathophysiologic Implications" Journal of Clinical Medicine 8, no. 8: 1256. https://doi.org/10.3390/jcm8081256